Human calpain 2, (m/II) large subunit ELISA Kit | CAPN2 elisa kit

Human calpain 2, (m/II) large subunit, CAPN2 ELISA Kit

Cat. Num. AAA918727

• Gene Names: CAPN2; CANP2; mCANP; CANPL2; CANPml
• Reactivity: Human
• Synonyms: calpain 2; (m/II) large subunit; Human calpain 2; CAPN2 ELISA Kit; (m/II) large subunit (CAPN2) ELISA kit; CANP2; CANPL2; CANPml; FLJ39928; mCANP; M-calpain; calcium-activated neutral proteinase; large [catalytic] subunit; large subunit; calpain M-type; calpain; large polypepti; CAPN2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CAPN2. No significant cross-reactivity or interference between human CAPN2 and analogues was observed.
• Sequence Length: 622

• Samples: Serum, plasma, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml -10 ng/ml.
• Sensitivity: The minimum detectable dose of human CAPN2 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAPN2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAPN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAPN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAPN2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAPN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 225703100
• NCBI GeneID: 824
• NCBI Accession #: NP_001139540.1
• NCBI GenBank Nucleotide #: NM_001146068.1
• UniProt Accession #: P17655; Q16738; Q6PJT3; Q8WU26; Q9HBB1; A6NDG7; B7ZA96; E7ES58
• Molecular Weight: 71,476 Da
• NCBI Official Full Name: calpain-2 catalytic subunit isoform 2
• NCBI Official Synonym Full Names: calpain 2, (m/II) large subunit
• NCBI Official Symbol: CAPN2
• NCBI Official Synonym Symbols: CANP2; mCANP; CANPL2; CANPml
• NCBI Protein Information: calpain-2 catalytic subunit; CANP 2; M-calpain; calpain M-type; millimolar-calpain; calpain-2 large subunit; calpain 2, large subunit; calpain large polypeptide L2; calpain, large polypeptide L2; calpain 2, large [catalytic] subunit; calcium-activated neutral proteinase 2
• UniProt Protein Name: Calpain-2 catalytic subunit
• Protein Family: Calpain
• UniProt Gene Name: CAPN2
• UniProt Synonym Gene Names: CANPL2; CANP 2; M-calpain
• UniProt Entry Name: CAN2_HUMAN

NCBI Description

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

CAPN2: Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Ubiquitous. Activated by 200-1000 micromolar concentrations of calcium and inhibited by calpastatin. Belongs to the peptidase C2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Motility/polarity/chemotaxis; EC 3.4.22.53

Chromosomal Location of Human Ortholog: 1q41-q42

Cellular Component: Golgi apparatus; cortical actin cytoskeleton; focal adhesion; endoplasmic reticulum; cytoplasm; dendrite; plasma membrane; pseudopodium; nucleus; chromatin; cytosol; lipid raft

Molecular Function: protein binding; protein heterodimerization activity; cytoskeletal protein binding; calcium-dependent cysteine-type endopeptidase activity; calcium ion binding; cysteine-type peptidase activity

Biological Process: protein autoprocessing; blastocyst development; proteolysis involved in cellular protein catabolic process; response to hypoxia; proteolysis; myoblast fusion; regulation of cytoskeleton organization and biogenesis