Mouse tyrosine hydroxylase (TH) ELISA Kit | TH elisa kit

Mouse tyrosine hydroxylase, TH ELISA Kit

Cat. Num. AAA926829

• Reactivity: Mouse
• Synonyms: tyrosine hydroxylase (TH); Mouse tyrosine hydroxylase; TH ELISA Kit; Mouse tyrosine hydroxylase (TH) ELISA Kit; DYT14; DYT5b; TYH; dystonia 14; tyrosine 3-monooxygenase; TH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse TH. No significant cross-reactivity or interference between mouse TH and analogues was observed.
• Sequence Length: 498

• Samples: Serum, plasma, tissue homogenates, cell culture supernates
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: < 15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TH elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6678337
• NCBI GeneID: 21823
• NCBI Accession #: NP_033403.1
• NCBI GenBank Nucleotide #: NM_009377.1
• UniProt Accession #: P24529
• Molecular Weight: 55,993 Da
• NCBI Official Full Name: tyrosine 3-monooxygenase
• NCBI Official Synonym Full Names: tyrosine hydroxylase
• NCBI Official Symbol: Th
• NCBI Protein Information: tyrosine 3-monooxygenase; tyrosine 3-hydroxylase
• UniProt Protein Name: Tyrosine 3-monooxygenase
• UniProt Gene Name: Th
• UniProt Synonym Gene Names: TH
• UniProt Entry Name: TY3H_MOUSE

Uniprot Description

TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.

Protein type: Amino Acid Metabolism - tyrosine; Endoplasmic reticulum; Vesicle; EC 1.14.16.2; Oxidoreductase; Mitochondrial

Cellular Component: internal side of plasma membrane; neuron projection; cytoplasmic vesicle membrane; smooth endoplasmic reticulum; mitochondrion; dendrite; melanosome membrane; terminal button; perikaryon; cytosol; synaptic vesicle; cell soma; axon; cytoplasm; cytoplasmic vesicle; nucleus

Molecular Function: protein domain specific binding; ferric iron binding; metal ion binding; ferrous iron binding; oxidoreductase activity; oxygen binding; tyrosine 3-monooxygenase activity; amino acid binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen; enzyme binding; iron ion binding; dopamine binding; monooxygenase activity

Biological Process: synaptic transmission, dopaminergic; eating behavior; heart development; mating behavior; locomotory behavior; learning; dopamine biosynthetic process; memory; synaptic vesicle amine transport; regulation of heart contraction; norepinephrine biosynthetic process; dopamine biosynthetic process from tyrosine; organ morphogenesis; eye photoreceptor cell development; response to ethanol; catecholamine biosynthetic process; visual perception; aromatic amino acid family metabolic process; response to hypoxia; embryonic camera-type eye morphogenesis; epinephrine biosynthetic process; neurotransmitter biosynthetic process