Typical Testing Data/Standard Curve (for reference only)
Human tyrosine hydroxylase ELISA Kit | TH elisa kit
Human tyrosine hydroxylase, TH ELISA Kit
Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Human
Synonyms
tyrosine hydroxylase; Human tyrosine hydroxylase; TH ELISA Kit; DYT14; DYT5b; TYH; dystonia 14; tyrosine 3-monooxygenase; TH elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human TH. No significant cross-reactivity or interference between human TH and analogues was observed.
Sequence Length
497
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
< 0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TH elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TH bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,600 Da
NCBI Official Full Name
tyrosine 3-monooxygenase isoform b
NCBI Official Synonym Full Names
tyrosine hydroxylase
NCBI Official Symbol
TH
NCBI Official Synonym Symbols
TYH; DYT14; DYT5b
NCBI Protein Information
tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase
UniProt Protein Name
Tyrosine 3-monooxygenase
UniProt Gene Name
TH
UniProt Synonym Gene Names
TYH; TH
UniProt Entry Name
TY3H_HUMAN
NCBI Description
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Plays an important role in the physiology of adrenergic neurons.
Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.
Cofactor: Fe2+ ion.
Enzyme regulation: Phosphorylation leads to an increase in the catalytic activity.
Research Articles on TH
Similar Products
Product Notes
The Human TH th (Catalog #AAA701568) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701568 ELISA Kit recognizes Human TH. It is sometimes possible for the material contained within the vial of "tyrosine hydroxylase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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