p21Cip1 (S78) Blocking Peptide | Cdkn1a blocking peptide

Phospho-mouse p21Cip1(S78) Blocking Peptide

• Synonyms: p21Cip1 (S78); Phospho-mouse p21Cip1(S78) Blocking Peptide; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein; p21; Cdkn1a; Cip1; Waf1; Cdkn1a blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 72-85 of MOUSE Cdkn1a
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 211

• Cellular Location: Cytoplasm. Nucleus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Cdkn1a blocking peptide

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 209862835
• NCBI GeneID: 896
• NCBI Accession #: NP_001129489.1
• NCBI GenBank Nucleotide #: NM_001136017.3
• UniProt Accession #: P39689; P30281
• Molecular Weight: 17,785 Da
• NCBI Official Full Name: G1/S-specific cyclin-D3 isoform 1
• NCBI Official Synonym Full Names: cyclin D3
• NCBI Official Symbol: CCND3
• NCBI Protein Information: G1/S-specific cyclin-D3
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1
• UniProt Gene Name: Cdkn1a
• UniProt Synonym Gene Names: Cip1; Waf1
• UniProt Entry Name: CDN1A_MOUSE

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.

Protein type: Cell cycle regulation; Inhibitor

Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytoplasm; cytosol; nucleus; perinuclear region of cytoplasm

Molecular Function: cyclin binding; cyclin-dependent protein kinase activating kinase activity; cyclin-dependent protein kinase inhibitor activity; protein binding; protein complex binding; ubiquitin protein ligase binding

Biological Process: cell cycle arrest; cellular response to amino acid starvation; cellular response to extracellular stimulus; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of cyclin-dependent protein kinase activity; negative regulation of phosphorylation; positive regulation of B cell proliferation; positive regulation of fibroblast proliferation; positive regulation of programmed cell death; regulation of cell cycle; regulation of cyclin-dependent protein kinase activity; regulation of mitotic cell cycle; regulation of protein import into nucleus, translocation; response to DNA damage stimulus; response to UV