Mouse ADAM metallopeptidase domain 33 ELISA Kit | ADAM33 elisa kit

Mouse Disintegrin and metalloproteinase domain-containing protein 33, ADAM33 ELISA Kit

Cat. Num. AAA922898

• Gene Names: Adam33; Adaml
• Reactivity: Mouse
• Synonyms: ADAM metallopeptidase domain 33; Mouse Disintegrin and metalloproteinase domain-containing protein 33; ADAM33 ELISA Kit; Mouse Disintegrin and metalloproteinase domain-containing protein 33 (ADAM33) ELISA kit; RP5-964F7.2; C20orf153; DJ964F7.1; DKFZp434K0521; FLJ35308; FLJ36751; MGC149823; MGC71889; a disintegrin and metalloprotease 33; a disintegrin and metalloproteinase domain 33; disintegrin and reprolys; ADAM33 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse ADAM33. No significant cross-reactivity or interference between mouse ADAM33 and analogues was observed.
• Sequence Length: 797

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml -1000 pg/ml.
• Sensitivity: The minimum detectable dose of mouse ADAM33 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM33 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM33 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM33 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM33 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 254587924
• NCBI GeneID: 110751
• NCBI Accession #: NP_291093.2
• NCBI GenBank Nucleotide #: NM_033615.2
• UniProt Accession #: Q923W9; Q8R5G5
• Molecular Weight: 86,971 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 33 isoform 1
• NCBI Official Synonym Full Names: a disintegrin and metallopeptidase domain 33
• NCBI Official Symbol: Adam33
• NCBI Official Synonym Symbols: Adaml
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 33; ADAM 33; a disintegrin and metalloprotease domain 33
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 33
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: Adam33
• UniProt Synonym Gene Names: ADAM 33
• UniProt Entry Name: ADA33_MOUSE

Uniprot Description

ADAM33: Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA). The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; EC 3.4.24.-; Protease

Cellular Component: membrane; integral to membrane

Molecular Function: peptidase activity; hydrolase activity; zinc ion binding; metallopeptidase activity; metal ion binding; metalloendopeptidase activity

Biological Process: proteolysis; regulation of cytokine biosynthetic process; regulation of cell proliferation