RECK blocking peptide

RECK Antibody (Center) Blocking Peptide

• Gene Names: RECK; ST15
• Synonyms: RECK; RECK Antibody (Center) Blocking Peptide; Reversion-inducing cysteine-rich protein with Kazal motifs; hRECK; Suppressor of tumorigenicity 15 protein; ST15; RECK blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human RECK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 971

• Cellular Location: Cell membrane; Lipid-anchor, GPI-anchor.
• Tissue Location: Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RECK blocking peptide

Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.

NCBI and Uniprot Product Information

• NCBI GI #: 20178043
• NCBI GeneID: 8434
• UniProt Accession #: O95980; Q5W0K6; Q8WX37; B2RNS1
• Molecular Weight: 106,457 Da
• NCBI Official Full Name: Reversion-inducing cysteine-rich protein with Kazal motifs
• NCBI Official Synonym Full Names: reversion inducing cysteine rich protein with kazal motifs
• NCBI Official Symbol: RECK
• NCBI Official Synonym Symbols: ST15
• NCBI Protein Information: reversion-inducing cysteine-rich protein with Kazal motifs
• UniProt Protein Name: Reversion-inducing cysteine-rich protein with Kazal motifs
• Protein Family: Reversion-inducing cysteine-rich protein
• UniProt Gene Name: RECK
• UniProt Synonym Gene Names: ST15; hRECK
• UniProt Entry Name: RECK_HUMAN

NCBI Description

The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

RECK: Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression. Interacts with MMP-9. Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.

Protein type: Membrane protein, GPI anchor; Tumor suppressor

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: membrane

Molecular Function: endopeptidase inhibitor activity; metalloendopeptidase inhibitor activity; protein binding

Biological Process: extracellular matrix organization and biogenesis; negative regulation of cell migration