Western Blot (WB)
(Western blot analysis of RECK using Jurkat whole cell lysates)
Rabbit anti-Human, Mouse RECK Polyclonal Antibody | anti-RECK antibody
RECK Antibody
Gene Names
RECK; ST15
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RECK; Polyclonal Antibody; RECK Antibody; hRECK; Membrane anchored glycoprotein (metastasis and invasion); RECK protein; RECK_HUMAN; Reversion inducing cysteine rich protein with Kazal motifs; Reversion-inducing cysteine-rich protein with Kazal motifs; ST15; Suppression of tumorigenicity 15 (reversion inducing cysteine rich protein with kazal motifs); Suppressor of tumorigenicity 15; Suppressor of tumorigenicity 15 protein; anti-RECK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
RECK antibody detects endogenous levels of total RECK
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
971
Applicable Applications for anti-RECK antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human RECK
Subcellular Location
Plasma Membrane
Tissue Specificity
Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of RECK using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis of RECK using Jurkat whole cell lysates)
Related Product Information for anti-RECK antibody
Function: Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.
Subunit Structure: Interacts with MMP-9.
Post-translational Modifications: N-glycosylated.
Subunit Structure: Interacts with MMP-9.
Post-translational Modifications: N-glycosylated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 106 kDa
Predicted: 107 kDa
Predicted: 107 kDa
NCBI Official Full Name
reversion-inducing cysteine-rich protein with Kazal motifs isoform 1
NCBI Official Synonym Full Names
reversion inducing cysteine rich protein with kazal motifs
NCBI Official Symbol
RECK
NCBI Official Synonym Symbols
ST15
NCBI Protein Information
reversion-inducing cysteine-rich protein with Kazal motifs
UniProt Protein Name
Reversion-inducing cysteine-rich protein with Kazal motifs
Protein Family
UniProt Gene Name
RECK
UniProt Synonym Gene Names
ST15; hRECK
NCBI Description
The protein encoded by this gene is a cysteine-rich, extracellular protein with protease inhibitor-like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane-anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase-9, a key enzyme involved in tumor invasion and metastasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
Uniprot Description
Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.
Research Articles on RECK
Similar Products
Product Notes
The RECK reck (Catalog #AAA9609089) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RECK Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RECK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the RECK reck for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RECK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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