E2F5 blocking peptide

E2F5 Antibody (N-term) Blocking Peptide

• Gene Names: E2F5; E2F-5
• Synonyms: E2F5; E2F5 Antibody (N-term) Blocking Peptide; Transcription factor E2F5; E2F-5; E2F5 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human E2F5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 346

• Cellular Location: Nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for E2F5 blocking peptide

Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor- initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 2494230
• NCBI GeneID: 1875
• UniProt Accession #: Q15329; Q16601; Q92756; E9PBN9
• Molecular Weight: 20,192 Da
• NCBI Official Full Name: Transcription factor E2F5
• NCBI Official Synonym Full Names: E2F transcription factor 5
• NCBI Official Symbol: E2F5
• NCBI Official Synonym Symbols: E2F-5
• NCBI Protein Information: transcription factor E2F5
• UniProt Protein Name: Transcription factor E2F5
• Protein Family: Transcription factor
• UniProt Gene Name: E2F5
• UniProt Synonym Gene Names: E2F-5
• UniProt Entry Name: E2F5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F5: a member of the E2F/DP family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4 than to other E2F/DP family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: nucleolus; nucleoplasm; nucleus

Molecular Function: protein binding; transcription factor binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter