Human Mannose Binding Lectin (MBL) ELISA Kit | MBL elisa kit

Human Mannose Binding Lectin (MBL) ELISA Kit

Cat. Num. AAA269560

• Reactivity: Human
• Synonyms: Mannose Binding Lectin (MBL); Human Mannose Binding Lectin (MBL) ELISA Kit; MBL elisa kit

• Reactivity: Human
• Sequence Length: 38

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MBL elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human MBL monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 169335948
• Molecular Weight: 3,557 Da
• NCBI Official Full Name: mannose binding lectin, partial
• UniProt Protein Name: Mannose binding lectin
• Protein Family: MreB-like protein
• UniProt Gene Name: MBL2
• UniProt Entry Name: B1PN75_HUMAN

NCBI Description

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; cell surface; collagen; extracellular region

Molecular Function: mannose binding; protein binding; calcium ion binding; calcium-dependent protein binding; receptor binding

Biological Process: defense response to Gram-positive bacterium; killing by host of symbiont cells; positive regulation of phagocytosis; defense response to bacterium; acute-phase response; innate immune response; response to oxidative stress; opsonization; complement activation, lectin pathway; complement activation, classical pathway; negative regulation of viral reproduction; complement activation

Disease: Mannose-binding Protein Deficiency