Western Blot (WB)
(Anti-Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin / HA Protein rabbit polyclonal antibody at 1:1000 dilution. Sample：Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin / HA Recombinant Protein Lane A: 5ng Lane B: 1ng Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.)
Rabbit Influenza A H7N7 Hemagglutinin/HA Polyclonal Antibody | anti-HA antibody
Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin/HA Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
H7N7
Applications
Western Blot, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
Influenza A H7N7 Hemagglutinin/HA; Polyclonal Antibody; Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin/HA Antibody; Rabbit PAb; Antigen Affinity Purified; Harvey rat sarcoma viral oncogene homolog; anti-HA antibody
Host
Rabbit
Reactivity
H7N7
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin/HA
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-HA antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-1:5000
ELISA: 1:5000-1:10,000
Antibody's applications haven't been validated with corresponding virus positive samples.
ELISA: 1:5000-1:10,000
Antibody's applications haven't been validated with corresponding virus positive samples.
Immunogen
Recombinant Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin/HA Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin/HA. Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin/HA specific IgG was purified by Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin/HA affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin / HA Protein rabbit polyclonal antibody at 1:1000 dilution. Sample：Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin / HA Recombinant Protein Lane A: 5ng Lane B: 1ng Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.)
Western Blot (WB)
(Anti-Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin / HA Protein rabbit polyclonal antibody at 1:1000 dilution. Sample：Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin / HA Recombinant Protein Lane A: 5ng Lane B: 1ng Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions.)
Related Product Information for anti-HA antibody
The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
References
White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton, Influenza hemagglutinin membrane anchor, PNAS, 2018
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton, Influenza hemagglutinin membrane anchor, PNAS, 2018
NCBI and Uniprot Product Information
Protein Family
Similar Products
Product Notes
The HA (Catalog #AAA8104831) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Influenza A H7N7 (A/Netherlands/219/2003) Hemagglutinin/HA Antibody, Rabbit PAb, Antigen Affinity Purified reacts with H7N7 and may cross-react with other species as described in the data sheet. AAA Biotech's Influenza A H7N7 Hemagglutinin/HA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-1:5000 ELISA: 1:5000-1:10,000 Antibody's applications haven't been validated with corresponding virus positive samples. Researchers should empirically determine the suitability of the HA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Influenza A H7N7 Hemagglutinin/HA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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