CSNK1D blocking peptide

CSNK1D Antibody (C-term) Blocking peptide

• Gene Names: CSNK1D; ASPS; HCKID; FASPS2; CKIdelta
• Synonyms: CSNK1D; CSNK1D Antibody (C-term) Blocking peptide; Casein kinase I isoform delta; CKI-delta; CKId; Tau-protein kinase CSNK1D; HCKID; CSNK1D blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human CK1d. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 415

• Cellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, cytoskeleton, spindle. Golgi apparatus. Note: Localized at mitotic spindle microtubules, and at the centrosomes and interphase in interphase cells. Recruited to the spindle apparatus and the centrosomes in response to DNA- damage. Correct subcellular localization requires kinase activity
• Tissue Location: Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. However, kinase activity is not uniform, with highest kinase activity in splenocytes. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CSNK1D blocking peptide

Essential serine/threonine-protein kinase that regulates diverse cellular growth and survival processes including Wnt signaling, DNA repair and circadian rhythms. It can phosphorylate a large number of proteins. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Phosphorylates connexin-43/GJA1, MAP1A, SNAPIN, MAPT/TAU, TOP2A, DCK, HIF1A, EIF6, p53/TP53, DVL2, DVL3, ESR1, AIB1/NCOA3, DNMT1, PKD2, YAP1, PER1 and PER2. Central component of the circadian clock. In balance with PP1, determines the circadian period length through the regulation of the speed and rhythmicity of PER1 and PER2 phospohorylation. Controls PER1 and PER2 nuclear transport and degradation. YAP1 phosphorylation promotes its SCF(beta-TRCP) E3 ubiquitin ligase-mediated ubiquitination and subsequent degradation. DNMT1 phosphorylation reduces its DNA-binding activity. Phosphorylation of ESR1 and AIB1/NCOA3 stimulates their activity and coactivation. Phosphorylation of DVL2 and DVL3 regulates WNT3A signaling pathway that controls neurite outgrowth. EIF6 phosphorylation promotes its nuclear export. Triggers down-regulation of dopamine receptors in the forebrain. Activates DCK in vitro by phosphorylation. TOP2A phosphorylation favors DNA cleavable complex formation. May regulate the formation of the mitotic spindle apparatus in extravillous trophoblast. Modulates connexin-43/GJA1 gap junction assembly by phosphorylation. Probably involved in lymphocyte physiology. Regulates fast synaptic transmission mediated by glutamate.

NCBI and Uniprot Product Information

• NCBI GI #: 27923980
• NCBI GeneID: 1453
• UniProt Accession #: P48730; Q96KZ6; Q9BTN5; A2I2P2
• Molecular Weight: 46,832 Da
• NCBI Official Full Name: Casein kinase I isoform delta
• NCBI Official Synonym Full Names: casein kinase 1 delta
• NCBI Official Symbol: CSNK1D
• NCBI Official Synonym Symbols: ASPS; HCKID; FASPS2; CKIdelta
• NCBI Protein Information: casein kinase I isoform delta
• UniProt Protein Name: Casein kinase I isoform delta
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1D
• UniProt Synonym Gene Names: HCKID; CKI-delta; CKId
• UniProt Entry Name: KC1D_HUMAN

NCBI Description

This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CK1D: an ubiquitous protein kinase of the CK1 family. Enriched at the centrosomes in interphase and at the spindle during mitosis. May play a role in cell cycle progression. Two splice variant isoforms have been described.

Protein type: Protein kinase, CK1; EC 2.7.11.26; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: centrosome; cytosol; ER-Golgi intermediate compartment membrane; Golgi apparatus; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; spindle; spindle microtubule

Molecular Function: protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: circadian regulation of gene expression; COPII coating of Golgi vesicle; DNA repair; endocytosis; G2/M transition of mitotic cell cycle; Golgi organization and biogenesis; microtubule nucleation; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; protein localization in Golgi apparatus; regulation of cell shape; regulation of circadian rhythm; rRNA processing; sensory cilium biogenesis; signal transduction; spindle assembly

Disease: Advanced Sleep Phase Syndrome, Familial, 2