Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysateAll lanes: CSNK1D antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 48,47 KDaObserved band size: 48 KDa)
Rabbit anti-Human CSNK1D Polyclonal Antibody | anti-CSNK1D antibody
CSNK1D Antibody
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysateAll lanes: CSNK1D antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 48,47 KDaObserved band size: 48 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: Hela whole cell lysateAll lanes: CSNK1D antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 48,47 KDaObserved band size: 48 KDa)
Immunohistochemistry (IHC)
(IHC image of MBS7002755 diluted at 1:1000 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7002755 diluted at 1:1000 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(Immunofluorescence staining of HepG2 cells with MBS7002755 at 1:333,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunohistochemistry (IHC)
(Immunofluorescence staining of HepG2 cells with MBS7002755 at 1:333,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunoprecipitation (IP)
(Immunoprecipitating CSNK1D in Hela whole cell lysate Lane 1: Rabbit control IgG (1ug)instead of MBS7002755 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7002755 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
Immunoprecipitation (IP)
(Immunoprecipitating CSNK1D in Hela whole cell lysate Lane 1: Rabbit control IgG (1ug)instead of MBS7002755 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7002755 (6ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug))
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
Uniprot Description
CK1D: an ubiquitous protein kinase of the CK1 family. Enriched at the centrosomes in interphase and at the spindle during mitosis. May play a role in cell cycle progression. Two splice variant isoforms have been described.
Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.26; Protein kinase, CK1; EC 2.7.11.1; Kinase, protein; CK1 group; CK1 family
Chromosomal Location of Human Ortholog: 17q25
Cellular Component: centrosome; cytosol; ER-Golgi intermediate compartment membrane; Golgi apparatus; Golgi membrane; neuron projection; nucleus; perinuclear region of cytoplasm; plasma membrane; spindle; spindle microtubule
Molecular Function: ATP binding; glycoprotein binding; peptide binding; phosphoprotein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; tau-protein kinase activity
Biological Process: cellular protein metabolic process; circadian regulation of gene expression; COPII coating of Golgi vesicle; DNA repair; ER to Golgi vesicle-mediated transport; G2/M transition of mitotic cell cycle; Golgi organization and biogenesis; microtubule nucleation; mitotic cell cycle; organelle organization and biogenesis; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; protein amino acid phosphorylation; protein localization in Golgi apparatus; regulation of circadian rhythm; sensory cilium biogenesis; signal transduction; spindle assembly; Wnt receptor signaling pathway
Disease: Advanced Sleep Phase Syndrome, Familial, 2
Research Articles on CSNK1D
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Product Notes
The CSNK1D csnk1d (Catalog #AAA7002755) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CSNK1D Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CSNK1D can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC). Recommended dilution: IHC: 1:20-1:200. Researchers should empirically determine the suitability of the CSNK1D csnk1d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSNK1D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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