DAPK2 blocking peptide

DAPK2 Antibody (N-term) Blocking Peptide

• Gene Names: DAPK2; DRP1; DRP-1
• Synonyms: DAPK2; DAPK2 Antibody (N-term) Blocking Peptide; Death-associated protein kinase 2; DAP kinase 2; DAP-kinase-related protein 1; DRP-1; DAPK2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of humanPK2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 370

• Cellular Location: Cytoplasm. Cytoplasmic vesicle, autophagosome lumen
• Tissue Location: Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. It is expressed abundantly in cells differentiated toward granulocytes and low in undifferentiated, normal and leukemic hematopoietic cells and monocytes/macrophages
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DAPK2 blocking peptide

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation.

NCBI and Uniprot Product Information

• NCBI GI #: 38605084
• NCBI GeneID: 23604
• UniProt Accession #: Q9UIK4; O75892; Q24JS1; E9JGM7
• Molecular Weight: 55,931 Da
• NCBI Official Full Name: Death-associated protein kinase 2
• NCBI Official Synonym Full Names: death associated protein kinase 2
• NCBI Official Symbol: DAPK2
• NCBI Official Synonym Symbols: DRP1; DRP-1
• NCBI Protein Information: death-associated protein kinase 2
• UniProt Protein Name: Death-associated protein kinase 2
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK2
• UniProt Synonym Gene Names: DAP kinase 2; DRP-1
• UniProt Entry Name: DAPK2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008]

Uniprot Description

DAPK2: an ubiquitously expressed protein kinase of the DAPK family. Activated by Ca(2+)/calmodulin. Contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain. Overexpression induces apoptosis. Expressed at high levels in heart, lung and skeletal muscle.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: cytoplasm

Molecular Function: ATP binding; calmodulin binding; identical protein binding; protein binding; protein serine/threonine kinase activity

Biological Process: anoikis; apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of autophagy