EMD blocking peptide

EMD Antibody (C-term) Blocking Peptide

• Gene Names: EMD; STA; EDMD; LEMD5
• Synonyms: EMD; EMD Antibody (C-term) Blocking Peptide; Emerin; EDMD; STA; EMD blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human EMD. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 254

• Cellular Location: Nucleus inner membrane; Single-pass membrane protein; Nucleoplasmic side. Nucleus outer membrane Note: Colocalized with BANF1 at the central region of the assembling nuclear rim, near spindle-attachment sites. The accumulation of different intermediates of prelamin-A/C (non- farnesylated or carboxymethylated farnesylated prelamin-A/C) in fibroblasts modify its localization in the nucleus
• Tissue Location: Skeletal muscle, heart, colon, testis, ovary and pancreas
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EMD blocking peptide

Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta- catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.

NCBI and Uniprot Product Information

• NCBI GI #: 1706639
• NCBI GeneID: 2010
• UniProt Accession #: P50402; Q6FI02
• Molecular Weight: 28,994 Da
• NCBI Official Full Name: Emerin
• NCBI Official Synonym Full Names: emerin
• NCBI Official Symbol: EMD
• NCBI Official Synonym Symbols: STA; EDMD; LEMD5
• NCBI Protein Information: emerin
• UniProt Protein Name: Emerin
• Protein Family: Emerin
• UniProt Gene Name: EMD
• UniProt Synonym Gene Names: EDMD; STA
• UniProt Entry Name: EMD_HUMAN

NCBI Description

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.