Rabbit EMD Polyclonal Antibody | anti-EMD antibody

EMD Antibody

Cat. Num. AAA9409653

• Gene Names: EMD; STA; EDMD; LEMD5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: EMD; Polyclonal Antibody; EMD Antibody; EDMD; LEMD5; STA; anti-EMD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total EMD protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 254

• Applicable Applications for anti-EMD antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100; Immunofluorescence: 1:20 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human EMD.
• Target Name: EMD
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using EMD antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human thyroid cancer using EMD antibody at dilution of 1:200 (400x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using EMD antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-EMD antibody

Emerin is a broadly expressed integral protein of the nuclear inner membrane (1). It contains a LEM domain and binds to several nuclear proteins, such as BAF (barrier-to-autointegration factor) and A- and B-type lamins, which are important in nuclear functions (2-5). Emerin may regulate gene expression through binding to other transcriptional regulators (6,7). Emerin binds to b-catenin and inhibits its nuclear accumulation (8). Recent studies demonstrate that Emerin is required for HIV-1 infectivity (9). Mutations in Emerin are a major cause of Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive skeletal muscle weakening (10).

Product Categories/Family for anti-EMD antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4557553
• NCBI GeneID: 2010
• NCBI Accession #: NP_000108.1
• NCBI GenBank Nucleotide #: NM_000117.2
• UniProt Accession #: P50402; Q6FI02
• Molecular Weight: 28,994 Da
• NCBI Official Full Name: emerin
• NCBI Official Synonym Full Names: emerin
• NCBI Official Symbol: EMD
• NCBI Official Synonym Symbols: STA; EDMD; LEMD5
• NCBI Protein Information: emerin
• UniProt Protein Name: Emerin
• Protein Family: Emerin
• UniProt Gene Name: EMD
• UniProt Synonym Gene Names: EDMD; STA
• UniProt Entry Name: EMD_HUMAN

NCBI Description

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.