ABL (Y251) Blocking Peptide | ABL2 blocking peptide

ABL Antibody (Y251) Blocking Peptide

• Gene Names: ABL2; ARG; ABLL
• Synonyms: ABL (Y251); ABL Antibody (Y251) Blocking Peptide; Abelson tyrosine-protein kinase 2; Abelson murine leukemia viral oncogene homolog 2; Abelson-related gene protein; Tyrosine-protein kinase ARG; ABL2; ABLL; ARG; ABL2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Y251 region of human ABL. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1182

• Cellular Location: Cytoplasm, cytoskeleton.
• Tissue Location: Widely expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ABL2 blocking peptide

Non-receptor tyrosine-protein kinase that plays an ABL1- overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin- bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

NCBI and Uniprot Product Information

• NCBI GI #: 1168268
• NCBI GeneID: 27
• UniProt Accession #: P42684; Q5T0X6; Q5W0C5; Q6NZY6; Q7Z301; A0M8X0; B7UEF2; B7UEF3; B7UEF4; B7UEF5
• Molecular Weight: 60,864 Da
• NCBI Official Full Name: Abelson tyrosine-protein kinase 2
• NCBI Official Synonym Full Names: ABL proto-oncogene 2, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL2
• NCBI Official Synonym Symbols: ARG; ABLL
• NCBI Protein Information: Abelson tyrosine-protein kinase 2
• UniProt Protein Name: Abelson tyrosine-protein kinase 2
• Protein Family: Abelson tyrosine-protein kinase
• UniProt Gene Name: ABL2
• UniProt Synonym Gene Names: ABLL; ARG
• UniProt Entry Name: ABL2_HUMAN

NCBI Description

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

Arg: a cytoplasmic tyrosine kinase of the Abl family. Expressed at the highest levels in mature B cells. Promotes catalase degradation in the oxidative stress response. Localizes to dynamic actin structures and may regulate cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; TK group; Abl family

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: actin cytoskeleton; cytosol; extrinsic to internal side of plasma membrane

Molecular Function: actin filament binding; actin monomer binding; magnesium ion binding; manganese ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein-tyrosine kinase activity; receptor binding

Biological Process: elevation of cytosolic calcium ion concentration; innate immune response; peptidyl-tyrosine phosphorylation; positive regulation of oxidoreductase activity; protein modification process; regulation of autophagy; regulation of cell adhesion; regulation of cell proliferation; regulation of endocytosis; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway