ABL2 recombinant protein

Recombinant ABL2 (279-546) protein

Cat. Num. AAA389382

• Gene Names: ABL2; ARG; ABLL
• Synonyms: ABL2; Recombinant ABL2 (279-546) protein; Abelson murine leukemia viral oncogene homolog 2; Tyrosine-protein kinase ARG; ABL2 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant ABL2 (279-546) protein is supplied in 25mM HEPESNaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant ABL2 (279-546) protein gel 10% SDS-PAGE with Coomassie blue staining MW: 32 kDa Purity: >85%)

Related Product Information for ABL2 recombinant protein

Short Description: Recombinant ABL2 (279-546) protein that includes amino acids 279-546 of human ABL2 protein (accession number NP_009298.1) with a N-terminal DYKDDDDK Tag. The molecular weight of the protein is 32 kDa.

Background: ABL2 is a non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. It also coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). In addition to, ABL2 can bind directly F-actin and regulate actin cytoskeletal structure through its F-actin-bundling activity. Which also involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35.

Product Categories/Family for ABL2 recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 6382062
• NCBI GeneID: 27
• NCBI Accession #: NP_009298.1
• NCBI GenBank Nucleotide #: NM_007314.3
• UniProt Accession #: P42684; Q5T0X6; Q5W0C5; Q6NZY6; Q7Z301; A0M8X0; B7UEF2; B7UEF3; B7UEF4; B7UEF5
• Molecular Weight: 60,864 Da
• NCBI Official Full Name: Abelson tyrosine-protein kinase 2 isoform b
• NCBI Official Synonym Full Names: ABL proto-oncogene 2, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL2
• NCBI Official Synonym Symbols: ARG; ABLL
• NCBI Protein Information: Abelson tyrosine-protein kinase 2; tyrosine-protein kinase ARG; abelson-related gene protein; c-abl oncogene 2, non-receptor tyrosine kinase; v-abl Abelson murine leukemia viral oncogene homolog 2
• UniProt Protein Name: Abelson tyrosine-protein kinase 2
• Protein Family: Abelson tyrosine-protein kinase
• UniProt Gene Name: ABL2
• UniProt Synonym Gene Names: ABLL; ARG
• UniProt Entry Name: ABL2_HUMAN

NCBI Description

This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

Arg: a cytoplasmic tyrosine kinase of the Abl family. Expressed at the highest levels in mature B cells. Promotes catalase degradation in the oxidative stress response. Localizes to dynamic actin structures and may regulate cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; Oncoprotein; TK group; Abl family

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: extrinsic to internal side of plasma membrane; lamellipodium; dendritic spine; cytosol; actin cytoskeleton; phagocytic cup

Molecular Function: actin monomer binding; actin filament binding; protein binding; manganese ion binding; protein-tyrosine kinase activity; magnesium ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding; protein kinase activity

Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; positive regulation of protein binding; alpha-beta T cell differentiation; cerebellum morphogenesis; platelet-derived growth factor receptor signaling pathway; signal transduction; regulation of apoptosis; Bergmann glial cell differentiation; elevation of cytosolic calcium ion concentration; regulation of endocytosis; visual learning; neuromuscular process controlling balance; positive regulation of oxidoreductase activity; regulation of cell adhesion; epidermal growth factor receptor signaling pathway; actin filament bundle formation; cell migration; positive regulation of I-kappaB kinase/NF-kappaB cascade; dendrite morphogenesis; protein modification process; substrate-bound cell migration, cell extension; phagocytosis; regulation of cell proliferation; negative regulation of Rho protein signal transduction; innate immune response; regulation of autophagy; negative regulation of cell-cell adhesion; neuron remodeling