ATP5B blocking peptide

ATP5B Antibody (Center) Blocking Peptide

• Gene Names: ATP5B; ATPMB; ATPSB; HEL-S-271
• Synonyms: ATP5B; ATP5B Antibody (Center) Blocking Peptide; ATP synthase subunit beta; mitochondrial; ATPMB; ATPSB; ATP5B blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human ATP5B. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 529

• Cellular Location: Mitochondrion. Mitochondrion inner membrane. Note: Peripheral membrane protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATP5B blocking peptide

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

NCBI and Uniprot Product Information

• NCBI GI #: 114549
• NCBI GeneID: 506
• UniProt Accession #: P06576; Q14283; A8K4X0
• Molecular Weight: 56,560 Da
• NCBI Official Full Name: ATP synthase subunit beta, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
• NCBI Official Symbol: ATP5B
• NCBI Official Synonym Symbols: ATPMB; ATPSB; HEL-S-271
• NCBI Protein Information: ATP synthase subunit beta, mitochondrial
• UniProt Protein Name: ATP synthase subunit beta, mitochondrial
• UniProt Gene Name: ATP5B
• UniProt Synonym Gene Names: ATPMB; ATPSB
• UniProt Entry Name: ATPB_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP5B: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Belongs to the ATPase alpha/beta chains family.

Protein type: EC 3.6.3.14; Hydrolase; Mitochondrial; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cell surface; membrane; mitochondrial matrix; mitochondrial membrane; mitochondrial proton-transporting ATP synthase complex; mitochondrion; nucleus; plasma membrane

Molecular Function: ATPase activity; hydrogen ion transporting ATPase activity, rotational mechanism; MHC class I protein binding; protein binding; transmembrane transporter activity

Biological Process: angiogenesis; ATP biosynthetic process; mitochondrial ATP synthesis coupled proton transport; mitochondrion organization and biogenesis; osteoblast differentiation; proton transport; regulation of intracellular pH