Bovine Corticotropin Releasing Hormone Receptor ELISA Kit | CRH-R elisa kit

Bovine Corticotropin Releasing Hormone Receptor ELISA Kit

Cat. Num. AAA750861

• Gene Names: CRHR1; CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRHR1f; CRF-R-1; CRH-R-1; CRH-R1h
• Reactivity: Bovine
• Synonyms: Corticotropin Releasing Hormone Receptor; Bovine Corticotropin Releasing Hormone Receptor ELISA Kit; CRH-R elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This CRH-R ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine CRH-R. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRH-R elisa kit

Principle of the Assay: CRH-R ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CRH-R antibody and an CRH-R-HRP conjugate. The assay sample and buffer are incubated together with CRH-R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRH-R concentration since CRH-R from samples and CRH-R-HRP conjugate compete for the anti-CRH-R antibody binding site. Since the number of sites is limited, as more sites are occupied by CRH-R from the sample, fewer sites are left to bind CRH-R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRH-R concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CRH-R elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 372466597
• NCBI GeneID: 1394
• NCBI Accession #: NP_001243228.1
• NCBI GenBank Nucleotide #: NM_001256299.1
• Molecular Weight: 28,122 Da
• NCBI Official Full Name: corticotropin-releasing factor receptor 1 isoform 5
• NCBI Official Synonym Full Names: corticotropin releasing hormone receptor 1
• NCBI Official Symbol: CRHR1
• NCBI Official Synonym Symbols: CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRHR1f; CRF-R-1; CRH-R-1; CRH-R1h
• NCBI Protein Information: corticotropin-releasing factor receptor 1; CRH receptor 1 variant B; seven transmembrane helix receptor; corticotropin-releasing hormone receptor 1; corticotropin-releasing factor type 1 receptor; corticotropin releasing hormone receptor variant 1e; corticotropin releasing hormone receptor variant 1g
• UniProt Protein Name: Corticotropin-releasing factor receptor 1
• UniProt Gene Name: CRHR1
• UniProt Synonym Gene Names: CRFR; CRFR1; CRHR; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1
• UniProt Entry Name: CRFR1_HUMAN

NCBI Description

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants, one of which represents a read-through transcript with the neighboring gene MGC57346. [provided by RefSeq, Jan 2012]

Uniprot Description

CRF-R1: a G-protein coupled receptor that specifically binds corticotropin-releasing hormone, a potent mediator of endocrine, autonomic, behavioral, and immune responses to stress. Four splice-variant isoforms have been described.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 2; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: multivesicular body; cell soma; apical part of cell; integral to plasma membrane; dendrite; plasma membrane; integral to membrane; trans-Golgi network; vesicle; intrinsic to plasma membrane

Molecular Function: protein binding; corticotropin-releasing hormone binding; corticotropin-releasing hormone receptor activity; protein complex binding; G-protein alpha-subunit binding; corticotrophin-releasing factor receptor activity

Biological Process: fear response; hypothalamus development; hormone-mediated signaling; adrenal gland development; adenylate cyclase activation; positive regulation of mast cell degranulation; behavioral response to ethanol; female pregnancy; memory; behavioral response to pain; general adaptation syndrome, behavioral process; elevation of cytosolic calcium ion concentration; epithelial cell differentiation; adrenocorticotropin hormone secretion; neuropeptide signaling pathway; behavioral response to cocaine; response to hypoxia; negative regulation of epinephrine secretion; visual learning; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; parturition; response to electrical stimulus