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Western Blot (WB) (Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution. Positive control: Line1: Hela Line2: HepG2 Line3: 293T)

Rabbit ATPB Monoclonal Antibody | anti-ATPB antibody

ATPB Antibody

Gene Names
ATP5F1B; ATP5B; ATPMB; ATPSB; HEL-S-271
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
ATPB; Monoclonal Antibody; ATPB Antibody; ATP 5B antibody; ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody; ATP synthase subunit beta mitochondrial antibody; ATP synthase subunit beta; mitochondrial antibody; atp5b antibody; ATPB antibody; ATPB_HUMAN antibody; ATPMB antibody; ATPSB antibody; Epididymis secretory protein Li 271 antibody; HEL-S-271 antibody; Mitochondrial ATP synthase beta subunit antibody; Mitochondrial ATP Synthase Subunit Beta antibody; Mitochondrial ATP synthetase beta subunit antibody; anti-ATPB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Monoclonal
Clone Number
JM10-90
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ATPB antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-5000
IHC: 1:50-1:200
ICC: 1:100-1:500
IP: 1:10-1:50
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution. Positive control: Line1: Hela Line2: HepG2 Line3: 293T)

Western Blot (WB) (Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution. Positive control: Line1: Hela Line2: HepG2 Line3: 293T)

Western Blot (WB)

(Western blot analysis of ATPB on Zebrafish cells lysates using anti-ATPB antibody at 1/500 dilution.)

Western Blot (WB) (Western blot analysis of ATPB on Zebrafish cells lysates using anti-ATPB antibody at 1/500 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded huaman uterus tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded huaman uterus tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATPB antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining ATPB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining ATPB in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-ATPB antibody
Mitochondrial membrane ATP synthase (F (1)F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F (1). Rotation of the central stalk against the surrounding alpha (3)beta (3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
Product Categories/Family for anti-ATPB antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
506
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56560 MW
NCBI Official Full Name
ATP synthase subunit beta, mitochondrial
NCBI Official Synonym Full Names
ATP synthase F1 subunit beta
NCBI Official Symbol
ATP5F1B
NCBI Official Synonym Symbols
ATP5B; ATPMB; ATPSB; HEL-S-271
NCBI Protein Information
ATP synthase subunit beta, mitochondrial
UniProt Protein Name
ATP synthase subunit beta, mitochondrial
Protein Family
UniProt Gene Name
ATP5B
UniProt Synonym Gene Names
ATPMB; ATPSB

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq, Jul 2008]

Uniprot Description

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Research Articles on ATPB

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Product Notes

The ATPB atp5b (Catalog #AAA9436187) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATPB Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's ATPB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 1:1000-5000 IHC: 1:50-1:200 ICC: 1:100-1:500 IP: 1:10-1:50. Researchers should empirically determine the suitability of the ATPB atp5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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