JMY blocking peptide

JMY Antibody(N-term) Blocking peptide

• Gene Names: JMY; WHAMM2; WHDC1L3
• Synonyms: JMY; JMY Antibody(N-term) Blocking peptide; Junction-mediating and -regulatory protein; JMY blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 988

• Cellular Location: Nucleus. Cytoplasm, cytoskeleton. Note: Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility (By similarity). In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for JMY blocking peptide

Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 172045777
• NCBI GeneID: 133746
• UniProt Accession #: Q8N9B5; A1L4P5; B5MDS2; B5MDT0
• Molecular Weight: 110,280 Da
• NCBI Official Full Name: Junction-mediating and -regulatory protein
• NCBI Official Synonym Full Names: junction mediating and regulatory protein, p53 cofactor
• NCBI Official Symbol: JMY
• NCBI Official Synonym Symbols: WHAMM2; WHDC1L3
• NCBI Protein Information: junction-mediating and -regulatory protein
• UniProt Protein Name: Junction-mediating and -regulatory protein
• Protein Family: Junction-mediating and -regulatory protein
• UniProt Gene Name: JMY
• UniProt Entry Name: JMY_HUMAN

Uniprot Description

JMY: Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location. Belongs to the JMY family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: leading edge; nucleoplasm; nucleus

Molecular Function: protein binding; transcription coactivator activity

Biological Process: cell cycle arrest; positive regulation of apoptosis; positive regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter