Rabbit anti-Human JMY Polyclonal Antibody | anti-JMY antibody

JMY, NT (JMY, Junction-mediating and-regulatory protein) (Azide free) (HRP)

Cat. Num. AAA6311767

• Gene Names: JMY; WHDC1L3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: JMY; Polyclonal Antibody; NT (JMY; Junction-mediating and-regulatory protein) (Azide free) (HRP); Junction-mediating and-regulatory protein; anti-JMY antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-JMY antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: JMY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human JMY.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(JMY Antibody (N-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the JMY antibody detected the JMY protein (arrow).)

Related Product Information for anti-JMY antibody

JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).

Product Categories/Family for anti-JMY antibody

Antibodies; Connexin; Junction Proteins

References

Coutts, A.S., et al. Proc. Natl. Acad. Sci. U.S.A. 106(47):19872-19877(2009). Zuchero, J.B., et al. Nat. Cell Biol. 11(4):451-459(2009). Nordgard, S.H., et al. Genes Chromosomes Cancer 47(8):680-696(2008). Coutts, A.S., et al. EMBO Rep. 8(1):84-90(2007). Shikama, N., et al. Mol. Cell 4(3):365-376(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 289547556
• NCBI GeneID: 133746
• NCBI Accession #: NP_689618.4
• NCBI GenBank Nucleotide #: NM_152405.4
• UniProt Accession #: Q8N9B5; A1L4P5; B5MDS2; B5MDT0
• Molecular Weight: 110,280 Da
• NCBI Official Full Name: junction-mediating and -regulatory protein
• NCBI Official Synonym Full Names: junction mediating and regulatory protein, p53 cofactor
• NCBI Official Symbol: JMY
• NCBI Official Synonym Symbols: WHDC1L3
• NCBI Protein Information: junction-mediating and -regulatory protein; junction-mediating and regulatory protein; WAS protein homology region 2 domain containing 1-like 3
• UniProt Protein Name: Junction-mediating and -regulatory protein
• Protein Family: Junction-mediating and -regulatory protein
• UniProt Gene Name: JMY
• UniProt Entry Name: JMY_HUMAN

Uniprot Description

JMY: Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location. Belongs to the JMY family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: nucleoplasm; cytoskeleton; leading edge; cytoplasm; plasma membrane; cell junction; nucleus

Molecular Function: protein binding; transcription coactivator activity; actin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; positive regulation of apoptosis; positive regulation of transcription factor activity; cell cycle arrest; DNA repair