U2AF2 blocking peptide

U2AF2 Antibody (N-term) Blocking Peptide

• Gene Names: U2AF2; U2AF65
• Synonyms: U2AF2; U2AF2 Antibody (N-term) Blocking Peptide; Splicing factor U2AF 65 kDa subunit; U2 auxiliary factor 65 kDa subunit; hU2AF(65); hU2AF65; U2 snRNP auxiliary factor large subunit; U2AF65; U2AF2 blocking peptide

• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 475

• Cellular Location: Nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for U2AF2 blocking peptide

Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.

NCBI and Uniprot Product Information

• NCBI GI #: 267188
• NCBI GeneID: 11338
• UniProt Accession #: P26368; Q96HC5
• Molecular Weight: 53,121 Da
• NCBI Official Full Name: Splicing factor U2AF 65 kDa subunit
• NCBI Official Synonym Full Names: U2 small nuclear RNA auxiliary factor 2
• NCBI Official Symbol: U2AF2
• NCBI Official Synonym Symbols: U2AF65
• NCBI Protein Information: splicing factor U2AF 65 kDa subunit
• UniProt Protein Name: Splicing factor U2AF 65 kDa subunit
• Protein Family: Splicing factor
• UniProt Gene Name: U2AF2
• UniProt Synonym Gene Names: U2AF65; hU2AF(65); hU2AF65
• UniProt Entry Name: U2AF2_HUMAN

NCBI Description

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008]

Uniprot Description

U2AF2: Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: nuclear speck; nucleoplasm; nucleus; spliceosome

Molecular Function: enzyme binding; poly-pyrimidine tract binding; pre-mRNA 3'-splice site binding; protein binding

Biological Process: mRNA 3'-end processing; mRNA export from nucleus; mRNA processing; negative regulation of nuclear mRNA splicing, via spliceosome; nuclear mRNA splicing, via spliceosome; positive regulation of RNA splicing; RNA export from nucleus; termination of RNA polymerase II transcription