Rabbit anti-Human U2AF2 Polyclonal Antibody | anti-U2AF2 antibody

U2AF2 Antibody

Cat. Num. AAA7108123

• Gene Names: U2AF2; U2AF65
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: >95%, Protein G purified
• Synonyms: U2AF2; Polyclonal Antibody; U2AF2 Antibody; Splicing factor U2AF 65 kDa subunit; U2 auxiliary factor 65 kDa subunit; hU2AF(65); hU2AF65; U2 snRNP auxiliary factor large subunit; U2AF65; anti-U2AF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 471

• Applicable Applications for anti-U2AF2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:500-1:5000
• Immunogen: Recombinant Human Splicing factor U2AF 65 kDa subunit protein (124-246AA)
• Conjugation: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: HepG2 whole cell lysateAll lanes: U2AF2 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 54 KDaObserved band size: 54 KDa)

Related Product Information for anti-U2AF2 antibody

Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.

Product Categories/Family for anti-U2AF2 antibody

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 60279268
• NCBI GeneID: 11338
• NCBI Accession #: NP_001012496.1
• NCBI GenBank Nucleotide #: NM_001012478.1
• UniProt Accession #: P26368; Q96HC5
• Molecular Weight: 53,121 Da
• NCBI Official Full Name: splicing factor U2AF 65 kDa subunit isoform b
• NCBI Official Synonym Full Names: U2 small nuclear RNA auxiliary factor 2
• NCBI Official Symbol: U2AF2
• NCBI Official Synonym Symbols: U2AF65
• NCBI Protein Information: splicing factor U2AF 65 kDa subunit
• UniProt Protein Name: Splicing factor U2AF 65 kDa subunit
• Protein Family: Splicing factor
• UniProt Gene Name: U2AF2
• UniProt Synonym Gene Names: U2AF65; hU2AF(65); hU2AF65

NCBI Description

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008]

Uniprot Description

Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10.