Rabbit anti-Human, Mouse U2AF2 Polyclonal Antibody | anti-U2AF2 antibody

U2AF2 Polyclonal Antibody

Cat. Num. AAA127135

• Gene Names: U2AF2; U2AF65
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: U2AF2; Polyclonal Antibody; U2AF2 Polyclonal Antibody; U2AF65; anti-U2AF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 475

• Applicable Applications for anti-U2AF2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human U2AF2
• Calculated Molecular Weight: 53kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using U2AF2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using U2AF2 antibody. Blue: DAPI for nuclear staining.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 200ug extracts of SW620 cells using 1ug U2AF2 antibody. Western blot was performed from the immunoprecipitate using U2AF2 antibody at a dilition of 1:500.)

Related Product Information for anti-U2AF2 antibody

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.

Product Categories/Family for anti-U2AF2 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 267188
• NCBI GeneID: 11338
• UniProt Accession #: P26368; Q96HC5
• Molecular Weight: 475
• NCBI Official Full Name: Splicing factor U2AF 65 kDa subunit
• NCBI Official Synonym Full Names: U2 small nuclear RNA auxiliary factor 2
• NCBI Official Symbol: U2AF2
• NCBI Official Synonym Symbols: U2AF65
• NCBI Protein Information: splicing factor U2AF 65 kDa subunit; hU2AF65; U2 auxiliary factor 65 kDa subunit; U2 snRNP auxiliary factor large subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 small nuclear ribonucleoprotein auxiliary factor (65kD)
• UniProt Protein Name: Splicing factor U2AF 65 kDa subunit
• Protein Family: Splicing factor
• UniProt Gene Name: U2AF2
• UniProt Synonym Gene Names: U2AF65; hU2AF(65); hU2AF65
• UniProt Entry Name: U2AF2_HUMAN

NCBI Description

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq, Jul 2008]

Uniprot Description

U2AF2: Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Spliceosome; RNA splicing

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: nucleoplasm; spliceosome; nuclear speck

Molecular Function: protein binding; enzyme binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription; positive regulation of RNA splicing