GOPC blocking peptide

GOPC Antibody (C-term) Blocking peptide

• Gene Names: GOPC; CAL; FIG; PIST; GOPC1; dJ94G16.2
• Synonyms: GOPC; GOPC Antibody (C-term) Blocking peptide; Golgi-associated PDZ and coiled-coil motif-containing protein; CFTR-associated ligand; Fused in glioblastoma; PDZ protein interacting specifically with TC10; PIST; CAL; FIG; GOPC blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of GOPC. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 462

• Cellular Location: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendrite. Note: Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.
• Tissue Location: Ubiquitously expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GOPC blocking peptide

Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.

NCBI and Uniprot Product Information

• NCBI GI #: 74762751
• NCBI GeneID: 57120
• UniProt Accession #: Q9HD26; Q59FS4; Q969U8; A6NM30
• Molecular Weight: 35,130 Da
• NCBI Official Full Name: Golgi-associated PDZ and coiled-coil motif-containing protein
• NCBI Official Synonym Full Names: golgi associated PDZ and coiled-coil motif containing
• NCBI Official Symbol: GOPC
• NCBI Official Synonym Symbols: CAL; FIG; PIST; GOPC1; dJ94G16.2
• NCBI Protein Information: Golgi-associated PDZ and coiled-coil motif-containing protein
• UniProt Protein Name: Golgi-associated PDZ and coiled-coil motif-containing protein
• Protein Family: Golgi-associated PDZ and coiled-coil motif-containing protein
• UniProt Gene Name: GOPC
• UniProt Synonym Gene Names: CAL; FIG; PIST
• UniProt Entry Name: GOPC_HUMAN

NCBI Description

This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

PIST: a ubiquitously expressed peripheral membrane protein that contains a PDZ domain and two coiled-coil regions. Associates with the Golgi apparatus and plasma membrane, regulating intracellular protein trafficking and degradation. May play a role in autophagy, and regulate the intracellular trafficking of the ADRB1 receptor. Interacts with GOLGA3 and STX6. The PDZ domain is known to mediate interactions with CLCN3-iso2, ACCN3, CFTR, SSTR5, FDZ5, ADRB1, FZD8, GRID2, mGluR5, mGluR1. Mutations of PDZ residues S302D, T304E, K348D, K350E abrogates the ability of PIST to interact with the CFTR C terminus. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. An oncogenic fusion protein between PIST (aka FIG) and the receptor tyrosine kinase ROS is found in glioblastoma multiform. Unlike other fusion RTK oncogenes, he mechanism of activation of PIST-ROS does not appear to be dimerization. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. Three alternatively spliced human isoforms have been described.

Protein type: Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; Golgi apparatus; lysosomal membrane; membrane; plasma membrane; protein complex; trans-Golgi network transport vesicle

Molecular Function: protein binding

Biological Process: protein homooligomerization

Disease: Spermatogenic Failure 6