PIST Recombinant Protein | GOPC recombinant protein

PIST Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121784

• Gene Names: GOPC; CAL; FIG; PIST; GOPC1; dJ94G16.2
• Purity: >90% as determined by SDS-PAGE
• Synonyms: PIST; PIST Protein; Human; Recombinant (His Tag); Human GOPC/PIST Protein (His Tag); CAL Protein; dJ94G16.2 Protein; FIG Protein; GOPC1 Protein; golgi-associated PDZ and coiled-coil motif containing; GOPC recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% Glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ile286-Tyr462

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human GOPC (Q9HD26-1)(Ile286-Tyr462) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GOPC recombinant protein

Background: GOPC, also known as PIST, interacts specifically with TC1 (a Rho-family small GTPase)] as a binding partner for Rhotekin. Rhotekin associates with PIST in vitro and both polarized and non-polarized MDCK (Madin-Darby canine kidney) cells. The C-terminal SPV (Ser-Pro-Val) motif of Rhotekin binds to the PDZ domain of PIST. The co-localization of PIST and Rhotekin at the Golgi apparatus can be detected in non-polarized fibroblast-like MDCK cells and AJs (adherens junctions) in the fully polarized cells. PIST and Rhotekin are recruited from the cytosol to AJs as the cell becomes polarized. Expression of constitutively active Rho or prevention of Rhotekin-PIST interaction induced diffuse cytoplasmic distribution of Rhotekin in polarized MDCK cells. GOPC may regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. It may also regulate the intracellular trafficking of the ADR1B receptor. GOPC is ubiquitously expressed and its overexpression results in CFTR intracellular retention and degradation in the lysosomes.

References

Hicks SW. et al., 2005, J Biol Chem. 280 (32): 28944-51. Ito H. et al., 2006, Biochem J Aug. 397 (3): 389-98. Amin N. et al., 2012, Mol Psychiatry. 17 (11): 1116-29.

NCBI and Uniprot Product Information

• NCBI GI #: 74762751
• NCBI GeneID: 57120
• UniProt Accession #: Q9HD26; Q59FS4; Q969U8; A6NM30
• Molecular Weight: 462
• NCBI Official Full Name: Golgi-associated PDZ and coiled-coil motif-containing protein
• NCBI Official Synonym Full Names: golgi-associated PDZ and coiled-coil motif containing
• NCBI Official Symbol: GOPC
• NCBI Official Synonym Symbols: CAL; FIG; PIST; GOPC1; dJ94G16.2
• NCBI Protein Information: Golgi-associated PDZ and coiled-coil motif-containing protein; dJ94G16.2 PIST; fused in glioblastoma; CFTR-associated ligand; PDZ protein interacting specifically with TC10; Golgi associated PDZ and coiled-coil motif containing protein; golgi-associated P
• UniProt Protein Name: Golgi-associated PDZ and coiled-coil motif-containing protein
• Protein Family: Pistil-specific extensin-like protein
• UniProt Gene Name: GOPC
• UniProt Synonym Gene Names: CAL; FIG; PIST
• UniProt Entry Name: GOPC_HUMAN

NCBI Description

This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

PIST: a ubiquitously expressed peripheral membrane protein that contains a PDZ domain and two coiled-coil regions. Associates with the Golgi apparatus and plasma membrane, regulating intracellular protein trafficking and degradation. May play a role in autophagy, and regulate the intracellular trafficking of the ADRB1 receptor. Interacts with GOLGA3 and STX6. The PDZ domain is known to mediate interactions with CLCN3-iso2, ACCN3, CFTR, SSTR5, FDZ5, ADRB1, FZD8, GRID2, mGluR5, mGluR1. Mutations of PDZ residues S302D, T304E, K348D, K350E abrogates the ability of PIST to interact with the CFTR C terminus. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. An oncogenic fusion protein between PIST (aka FIG) and the receptor tyrosine kinase ROS is found in glioblastoma multiform. Unlike other fusion RTK oncogenes, he mechanism of activation of PIST-ROS does not appear to be dimerization. Rather, activation of the fused ROS kinase appears to depend upon translocation to the golgi apparatus: deletion of 2nd coiled-coil region, crucial for Golgi localization, appears to eliminate the transformation capacity of PIST-ROS. Three alternatively spliced human isoforms have been described.

Protein type: Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: Golgi membrane; Golgi apparatus; postsynaptic membrane; trans-Golgi network transport vesicle; protein complex; membrane; postsynaptic density; cytoplasm; dendrite; plasma membrane; cell junction

Molecular Function: protein C-terminus binding; protein binding; protein homodimerization activity; frizzled binding

Biological Process: Golgi to plasma membrane transport; protein transport; ER to Golgi vesicle-mediated transport; apical protein localization; regulation of catalytic activity; spermatid nuclear differentiation; protein homooligomerization; cytoplasmic sequestering of CFTR protein

Disease: Spermatogenic Failure 6