Rabbit anti-Human NGEF Polyclonal Antibody | anti-NGEF antibody

NGEF Antibody (C-term)

Cat. Num. AAA9216135

• Gene Names: NGEF; EPHEXIN; ARHGEF27
• Reactivity: Human
• Applications: Western Blot
• Synonyms: NGEF; Polyclonal Antibody; NGEF Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); Ephexin-1; Eph-interacting exchange protein; Neuronal guanine nucleotide exchange factor; anti-NGEF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This NGEF antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 669-704 amino acids from the C-terminal region of human NGEF.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 618

• Applicable Applications for anti-NGEF antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-NGEF Antibody (C-term) at 1:2000 dilutionLane 1: A431 whole cell lysatesLane 2: PC-3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 82 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-NGEF antibody

Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).

Product Categories/Family for anti-NGEF antibody

Neuroscience; Signal Transduction

References

Ota T.,et al.Nat. Genet. 36:40-45(2004). Hillier L.W.,et al.Nature 434:724-731(2005). Rodrigues N.R.,et al.Genomics 65:53-61(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 166197690
• NCBI GeneID: 25791
• NCBI Accession #: NP_001107562.1
• NCBI GenBank Nucleotide #: NM_001114090.1
• UniProt Accession #: Q8N5V2; Q53QQ4; Q53ST7; Q6GMQ5; Q9NQD6; B4DMB8; B9A045; E9PC42
• Molecular Weight: 82496
• NCBI Official Full Name: ephexin-1 isoform 2
• NCBI Official Synonym Full Names: neuronal guanine nucleotide exchange factor
• NCBI Official Symbol: NGEF
• NCBI Official Synonym Symbols: EPHEXIN; ARHGEF27
• NCBI Protein Information: ephexin-1
• UniProt Protein Name: Ephexin-1
• Protein Family: Ephexin
• UniProt Gene Name: NGEF
• UniProt Entry Name: NGEF_HUMAN

NCBI Description

ephexin-1: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension. Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase. Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: growth cone; membrane; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity

Biological Process: axon guidance; regulation of small GTPase mediated signal transduction; regulation of GTPase activity; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; ephrin receptor signaling pathway; regulation of Rho protein signal transduction; positive regulation of GTPase activity

Uniprot Description

ephexin-1: Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension. Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase. Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rac/Rho; GEFs

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: cytosol; growth cone; membrane

Molecular Function: guanyl-nucleotide exchange factor activity; Rho guanyl-nucleotide exchange factor activity

Biological Process: axon guidance; ephrin receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of GTPase activity; regulation of GTPase activity; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction