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SDS-Page (Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Rat brain tissue and Mouse brain tissue lysates, Primary antibody:47316(NGEF Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Rabbit NGEF Polyclonal Antibody | anti-NGEF antibody

NGEF Antibody

Gene Names
NGEF; EPHEXIN; ARHGEF27
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
NGEF; Polyclonal Antibody; NGEF Antibody; EPHEXIN; ARHGEF27; anti-NGEF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody detects endogenous levels of total NGEF protein.
Purity/Purification
Antigen affinity purification
Form/Format
pH 7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.6 mg/mL (varies by lot)
Sequence Length
618
Applicable Applications for anti-NGEF antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-2000
IHC: 1:30-150
Immunogen
Fusion protein of human NGEF
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C.

SDS-Page

(Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Rat brain tissue and Mouse brain tissue lysates, Primary antibody:47316(NGEF Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

SDS-Page (Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Rat brain tissue and Mouse brain tissue lysates, Primary antibody:47316(NGEF Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 47316(NGEF Antibody) at dilution 1/35.(Original magnification: 200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 47316(NGEF Antibody) at dilution 1/35.(Original magnification: 200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 47316(NGEF Antibody) at dilution 1/35.(Original magnification: 200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 47316(NGEF Antibody) at dilution 1/35.(Original magnification: 200))
Related Product Information for anti-NGEF antibody
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).
Product Categories/Family for anti-NGEF antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82 kDa
NCBI Official Full Name
ephexin-1 isoform 2
NCBI Official Synonym Full Names
neuronal guanine nucleotide exchange factor
NCBI Official Symbol
NGEF
NCBI Official Synonym Symbols
EPHEXIN; ARHGEF27
NCBI Protein Information
ephexin-1
UniProt Protein Name
Ephexin-1
Protein Family
UniProt Gene Name
NGEF

Uniprot Description

Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension ().

Research Articles on NGEF

Similar Products

Product Notes

The NGEF ngef (Catalog #AAA9430299) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NGEF Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NGEF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-2000 IHC: 1:30-150. Researchers should empirically determine the suitability of the NGEF ngef for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NGEF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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