Rabbit anti-Human TrkA Polyclonal Antibody | anti-TrkA antibody

TrkA (Y791) (High Affinity Nerve Growth Factor Receptor, Neurotrophic Tyrosine Kinase Receptor Type 1, TRK1 Transforming Tyrosine Kinase Protein, p140-TrkA, Trk-A, NTRK1, TRK) (APC)

Cat. Num. AAA6503654

• Gene Names: NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: TrkA; Polyclonal Antibody; TrkA (Y791) (High Affinity Nerve Growth Factor Receptor; Neurotrophic Tyrosine Kinase Receptor Type 1; TRK1 Transforming Tyrosine Kinase Protein; p140-TrkA; Trk-A; NTRK1; TRK) (APC); anti-TrkA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TrkA.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 796

• Applicable Applications for anti-TrkA antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the Y791 region of human TrkA (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hTrkA-pY791 in HepG2 cell line lysates (35ug/lane). TRK (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(TrkA-pY791 Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(TrkA-pY791 Antibody (T8663-01K) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Product Categories/Family for anti-TrkA antibody

Antibodies; Receptors; Tyrosine Kinase

References

1. Tokusashi, Y., et al., Int. J. Cancer 114(1):39-45 (2005). 2. Schulte, J.H., et al., Oncogene 24(1):165-177 (2005). 3. Frattini, M., et al., Oncogene 23(44):7436-7440 (2004). 4. Tacconelli, A., et al., Cancer Cell 6(4):347-360 (2004). 5. Florenes, V.A., et al., Am. J. Clin. Pathol. 122(3):412-420 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4585712
• NCBI GeneID: 4914
• NCBI Accession #: NP_002520
• NCBI GenBank Nucleotide #: NP_002520.2
• UniProt Accession #: P04629
• Molecular Weight: 87.497
• NCBI Official Full Name: high affinity nerve growth factor receptor isoform 2
• NCBI Official Synonym Full Names: neurotrophic receptor tyrosine kinase 1
• NCBI Official Symbol: NTRK1
• NCBI Official Synonym Symbols: MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• NCBI Protein Information: high affinity nerve growth factor receptor
• UniProt Protein Name: High affinity nerve growth factor receptor
• Protein Family: Trk system potassium uptake protein
• UniProt Gene Name: NTRK1
• UniProt Synonym Gene Names: MTC; TRK; TRKA; Trk-A
• UniProt Entry Name: NTRK1_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

Uniprot Description

TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.

Protein type: Protein kinase, TK; Oncoprotein; EC 2.7.10.1; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; Trk family

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: protein complex; cell surface; integral to plasma membrane; late endosome membrane; dendrite; early endosome; cell soma; early endosome membrane; axon; late endosome; plasma membrane; cytoplasmic vesicle; receptor complex; endosome

Molecular Function: neurotrophin p75 receptor binding; neurotrophin binding; protein binding; nerve growth factor receptor activity; protein homodimerization activity; ephrin receptor binding; nerve growth factor binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: circadian rhythm; axon guidance; mechanoreceptor differentiation; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; positive regulation of synaptic transmission, glutamatergic; protein amino acid phosphorylation; olfactory nerve development; activation of NF-kappaB transcription factor; negative regulation of cell proliferation; sympathetic nervous system development; response to radiation; learning and/or memory; small GTPase mediated signal transduction; response to axon injury; negative regulation of neuron apoptosis; response to electrical stimulus; detection of temperature stimulus involved in sensory perception of pain; response to hydrostatic pressure; aging; response to drug; response to nutrient levels; phosphoinositide-mediated signaling; adenylate cyclase activation; Sertoli cell development; detection of mechanical stimulus involved in sensory perception of pain; positive regulation of programmed cell death; positive regulation of angiogenesis; response to ethanol; phospholipase C activation; B cell differentiation; Ras protein signal transduction; response to activity; positive regulation of Ras protein signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Thyroid Carcinoma, Familial Medullary; Insensitivity To Pain, Congenital, With Anhidrosis