Rabbit anti-Human FHIT Polyclonal Antibody | anti-FHIT antibody

FHIT Antibody (N-Term)

Cat. Num. AAA9216060

• Gene Names: FHIT; FRA3B; AP3Aase
• Reactivity: Human
• Applications: Western Blot
• Synonyms: FHIT; Polyclonal Antibody; FHIT Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Bis(5'-adenosyl)-triphosphatase; AP3A hydrolase; AP3Aase; Diadenosine 5'; 5'''-P1; P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; anti-FHIT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This FHIT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between19-54 amino acids from human FHIT.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 147

• Applicable Applications for anti-FHIT antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-FHIT Antibody (N-Term) at 1:2000 dilutionLane 1: human kidney lysateLane 2: human liver lysateLane 3: HL-60 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 17 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-FHIT antibody

Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'- adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low- affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.

References

Ohta M.,et al.Cell 84:587-597(1996). Druck T.,et al.Cancer Res. 57:504-512(1997). Corominas R.,et al.Nat. Commun. 5:3650-3650(2014). Naqvi S.R.A.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 261278358
• NCBI GeneID: 2272
• NCBI Accession #: NP_001159715.1
• NCBI GenBank Nucleotide #: NM_001166243.1
• UniProt Accession #: P49789; Q45QG9; Q6IU12; A2IAS9; A2IAT0; A2IAT6; A8K1A9
• Molecular Weight: 16858
• NCBI Official Full Name: bis(5'-adenosyl)-triphosphatase
• NCBI Official Synonym Full Names: fragile histidine triad
• NCBI Official Symbol: FHIT
• NCBI Official Synonym Symbols: FRA3B; AP3Aase
• NCBI Protein Information: bis(5'-adenosyl)-triphosphatase
• UniProt Protein Name: Bis(5'-adenosyl)-triphosphatase
• Protein Family: Bis(5'-adenosyl)-triphosphatase
• UniProt Gene Name: FHIT
• UniProt Synonym Gene Names: AP3Aase
• UniProt Entry Name: FHIT_HUMAN

NCBI Description

This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

FHIT: a member of the histidine triad gene family. A diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. Its gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. A possible tumor suppressor in specific tissues. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. Aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas.

Protein type: Motility/polarity/chemotaxis; EC 3.6.1.29; DNA replication; Hydrolase; Nucleotide Metabolism - purine; Tumor suppressor

Chromosomal Location of Human Ortholog: 3p14.2

Cellular Component: mitochondrion; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; hydrolase activity; ubiquitin protein ligase binding; nucleotide binding; bis(5'-adenosyl)-triphosphatase activity; catalytic activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; nucleotide metabolic process; DNA replication; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; purine nucleotide metabolic process