Rabbit anti-Human RASGRP2 Polyclonal Antibody | anti-RASGRP2 antibody

RASGRP2 Antibody (N-term)

Cat. Num. AAA9215810

• Gene Names: RASGRP2; CDC25L; CALDAG-GEFI
• Reactivity: Human
• Applications: Western Blot
• Synonyms: RASGRP2; Polyclonal Antibody; RASGRP2 Antibody (N-term); Purified Rabbit Polyclonal Antibody (Pab); RAS guanyl-releasing protein 2; Calcium and DAG-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25-like protein; hCDC25L; F25B33 kinase-like protein; CDC25L; MCG7; anti-RASGRP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This RASGRP2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-142 amino acids from the N-terminal region of human RASGRP2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 609

• Applicable Applications for anti-RASGRP2 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-RASGRP2 Antibody (N-term) at 1:2000 dilution + human heart lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 69 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RASGRP2 antibody

Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.

Product Categories/Family for anti-RASGRP2 antibody

Signal Transduction

References

Kedra D.,et al.Hum. Genet. 100:611-619(1997). Kawasaki H.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:13278-13283(1998). Clyde-Smith J.,et al.J. Biol. Chem. 275:32260-32267(2000). Ota T.,et al.Nat. Genet. 36:40-45(2004). Taylor T.D.,et al.Nature 440:497-500(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 149158727
• NCBI GeneID: 10235
• NCBI Accession #: NP_001092140.1
• NCBI GenBank Nucleotide #: NM_001098670.1
• UniProt Accession #: Q7LDG7; O00538; Q9UL65; A6NDC7
• Molecular Weight: 69248
• NCBI Official Full Name: RAS guanyl-releasing protein 2
• NCBI Official Synonym Full Names: RAS guanyl releasing protein 2 (calcium and DAG-regulated)
• NCBI Official Symbol: RASGRP2
• NCBI Official Synonym Symbols: CDC25L; CALDAG-GEFI
• NCBI Protein Information: RAS guanyl-releasing protein 2
• UniProt Protein Name: RAS guanyl-releasing protein 2
• Protein Family: RAS guanyl-releasing protein
• UniProt Gene Name: RASGRP2
• UniProt Synonym Gene Names: CDC25L; MCG7; CalDAG-GEFI; hCDC25L
• UniProt Entry Name: GRP2_HUMAN

NCBI Description

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.