Dot Blot (DB)
(Dot blot analysis of Phospho-PI3KC3-S282 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit Phospho-PI3KC3 (S282) Polyclonal Antibody | anti-PIK3C3 antibody
Phospho-PI3KC3 (S282) Antibody
Dot Blot (DB)
(Dot blot analysis of Phospho-PI3KC3-S282 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Dot Blot (DB)
(Dot blot analysis of Phospho-PI3KC3-S282 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.
Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).
NCBI and Uniprot Product Information
Uniprot Description
PIK3C3: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive. Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Ubiquitously expressed, with a highest expression in skeletal muscle. Belongs to the PI3/PI4-kinase family.
Protein type: Kinase, lipid; Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.137; Motility/polarity/chemotaxis; Autophagy
Chromosomal Location of Human Ortholog: 18q12.3
Cellular Component: membrane; late endosome; midbody; phagocytic vesicle; axoneme; phosphoinositide 3-kinase complex; cytosol
Molecular Function: protein binding; 1-phosphatidylinositol-3-kinase activity; ATP binding; protein kinase activity
Biological Process: phosphoinositide-mediated signaling; endosome organization and biogenesis; cytokinesis; protein amino acid phosphorylation; response to leucine; regulation of protein secretion; early endosome to late endosome transport; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; protein processing; toll-like receptor 9 signaling pathway; autophagic vacuole formation
Research Articles on PIK3C3
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Product Notes
The PIK3C3 pik3c3 (Catalog #AAA9214628) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-PI3KC3 (S282) Antibody reacts with Human (Predicted Reactivity: Mouse, Rat, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-PI3KC3 (S282) can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), ELISA (EIA). DB~~1:500. Researchers should empirically determine the suitability of the PIK3C3 pik3c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-PI3KC3 (S282), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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