Rabbit anti-Human PI3KC3 Polyclonal Antibody | anti-PIK3C3 antibody

PI3KC3, phosphorylated (Ser282) (Phosphatidylinositol 3-kinase Catalytic Subunit Type 3, PtdIns-3-kinase Type 3, PI3-kinase Type 3, PI3K Type 3, Phosphoinositide-3-kinase Class 3, Phosphatidylinositol 3-Kinase p100 Subunit, hVps34, VPS34)

Cat. Num. AAA624519

• Gene Names: PIK3C3; VPS34; hVps34; MGC61518
• Reactivity: Human
• Applications: ELISA, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PI3KC3; Polyclonal Antibody; phosphorylated (Ser282) (Phosphatidylinositol 3-kinase Catalytic Subunit Type 3; PtdIns-3-kinase Type 3; PI3-kinase Type 3; PI3K Type 3; Phosphoinositide-3-kinase Class 3; Phosphatidylinositol 3-Kinase p100 Subunit; hVps34; VPS34); Anti -PI3KC3; anti-PIK3C3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PI3KC3 when phosphorylated at Ser282.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PIK3C3 antibody: ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in ELISA and Dot Blot.; Dilution: ELISA: 1:1,000; Dot Blot: 1:500
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser282 of human PI3KC3 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of Phospho-PI3KC3-S282 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-PIK3C3 antibody

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

Product Categories/Family for anti-PIK3C3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 34761064
• NCBI GeneID: 5289
• NCBI Accession #: NP_002638.2
• NCBI GenBank Nucleotide #: NM_002647.2
• UniProt Accession #: Q8NEB9; Q15134
• Molecular Weight: 101,549 Da
• NCBI Official Full Name: phosphatidylinositol 3-kinase catalytic subunit type 3
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, class 3
• NCBI Official Symbol: PIK3C3
• NCBI Official Synonym Symbols: VPS34; hVps34; MGC61518
• NCBI Protein Information: phosphatidylinositol 3-kinase catalytic subunit type 3; PI3K type 3; PI3-kinase type 3; OTTHUMP00000163416; PtdIns-3-kinase type 3; phosphatidylinositol 3-kinase p100 subunit
• UniProt Protein Name: Phosphatidylinositol 3-kinase catalytic subunit type 3
• UniProt Gene Name: PIK3C3
• UniProt Synonym Gene Names: VPS34
• UniProt Entry Name: PK3C3_HUMAN

Uniprot Description

PIK3C3: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive. Part of a complex composed of PIK3R4 and PIK3CB. Interacts with RAB7A in the presence of PIK3R4. Ubiquitously expressed, with a highest expression in skeletal muscle. Belongs to the PI3/PI4-kinase family.

Protein type: Autophagy; EC 2.7.1.137; Carbohydrate Metabolism - inositol phosphate; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18q12.3

Cellular Component: membrane; late endosome; phagocytic vesicle; axoneme; midbody; phosphoinositide 3-kinase complex; cytosol

Molecular Function: protein binding; 1-phosphatidylinositol-3-kinase activity; ATP binding; protein kinase activity

Biological Process: phosphoinositide-mediated signaling; endosome organization and biogenesis; cytokinesis; protein amino acid phosphorylation; regulation of protein secretion; response to leucine; early endosome to late endosome transport; phosphoinositide phosphorylation; phospholipid metabolic process; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; protein processing; toll-like receptor 9 signaling pathway; autophagic vacuole formation