Western Blot (WB)
(Figure 1: Western blot analysis using KLF15 mouse mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.)
Mouse anti-Human KLF15 Monoclonal Antibody | anti-KLF15 antibody
KLF15 Antibody
Gene Names
KLF15; KKLF
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Affinity-Chromatography
Synonyms
KLF15; Monoclonal Antibody; KLF15 Antibody; Kidney enriched Kruppel like factor; Kidney-enriched krueppel-like factor; KKLF; KKLF protein; KLF 15; KLF15_HUMAN; Krueppel-like factor 15; Kruppel like factor 15; anti-KLF15 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
KLF15 antibody detects endogenous levels of total KLF15
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
416
Applicable Applications for anti-KLF15 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
WB: 1:500-1:2000
Immunogen
Purified recombinant fragment of human KLF15 expressed in E. Coli
Subcellular Location
Nucleus.
Tissue Specificity
Highly expressed in liver, skeletal muscle, and kidney. Expressed in cardiomyocytes. Expression is highly reduced in cardiac tissue of patients with non-ischemic cardiomyopathy and aortic aneurysm, and in glomerular disease. Not expressed in bone marrow or lymphoid tissues.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using KLF15 mouse mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.)
Western Blot (WB)
(Figure 1: Western blot analysis using KLF15 mouse mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.)
Related Product Information for anti-KLF15 antibody
Description: KLF15 (Kruppel-like factor 15), also known as KKLF, DKFZp779M1320. Entrez Protein NP_054798. Sequence analysis predicted that the 416 amino acid KLF15 protein, which is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment.KLF15, a kidney-enriched Krueppel-like factor, is a transcriptonal activator that binds the CLCNKA promoter. KLF15, which is a nuclear protein, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. KLF15 is an important regulator of GLUT4 in both adipose and muscle tissues.
Function: Transcriptional regulator that binds to the GA element of the CLCNKA promoter. Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart (By similarity). Is a repressor of CTGF expression, involved in the control of cardiac fibrosis. It is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A and GATA4 (By similarity). Involved in podocyte differentiation (By similarity). Inhibits MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation.
Subunit Structure: Interacts with MYOCD and EP300.
Similarity: Belongs to the Sp1 C2H2-type zinc-finger protein family.
Function: Transcriptional regulator that binds to the GA element of the CLCNKA promoter. Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart (By similarity). Is a repressor of CTGF expression, involved in the control of cardiac fibrosis. It is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A and GATA4 (By similarity). Involved in podocyte differentiation (By similarity). Inhibits MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation.
Subunit Structure: Interacts with MYOCD and EP300.
Similarity: Belongs to the Sp1 C2H2-type zinc-finger protein family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 44 kDa
Predicted: 44 kDa
Predicted: 44 kDa
NCBI Official Full Name
Krueppel-like factor 15
NCBI Official Synonym Full Names
Kruppel like factor 15
NCBI Official Symbol
KLF15
NCBI Official Synonym Symbols
KKLF
NCBI Protein Information
Krueppel-like factor 15
UniProt Protein Name
Krueppel-like factor 15
Protein Family
UniProt Gene Name
KLF15
UniProt Synonym Gene Names
KKLF
Uniprot Description
Transcriptional regulator that binds to the GA element of the CLCNKA promoter. Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart (). Is a repressor of CTGF expression, involved in the control of cardiac fibrosis. It is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A and GATA4 (). Involved in podocyte differentiation (). Inhibits MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation.
Research Articles on KLF15
Similar Products
Product Notes
The KLF15 klf15 (Catalog #AAA9602734) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KLF15 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KLF15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the KLF15 klf15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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