Human acyloxyacyl hydrolase (neutrophil) ELISA Kit | AOAH elisa kit

Human Acyloxyacyl hydrolase, AOAH ELISA Kit

Cat. Num. AAA921316

• Reactivity: Human
• Synonyms: acyloxyacyl hydrolase (neutrophil); Human Acyloxyacyl hydrolase; AOAH ELISA Kit; Human Acyloxyacyl hydrolase (AOAH) ELISA kit; acyloxyacyl hydrolase; AOAH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human AOAH. No significant cross-reactivity or interference between human AOAH and analogues was observed.
• Sequence Length: 688

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: Typically less than 0.039 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AOAH elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AOAH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AOAH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AOAH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AOAH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 294610665
• NCBI GeneID: 313
• NCBI Accession #: NP_001170977.1
• NCBI GenBank Nucleotide #: NM_001177506.1
• UniProt Accession #: P28039; Q53F13; A4D1Y5; B7Z490
• Molecular Weight: 65,105 Da
• NCBI Official Full Name: acyloxyacyl hydrolase isoform 2 preproprotein
• NCBI Official Synonym Full Names: acyloxyacyl hydrolase (neutrophil)
• NCBI Official Symbol: AOAH
• NCBI Protein Information: acyloxyacyl hydrolase
• UniProt Protein Name: Acyloxyacyl hydrolase
• Protein Family: Acyloxyacyl hydrolase
• UniProt Gene Name: AOAH
• UniProt Entry Name: AOAH_HUMAN

NCBI Description

This locus encodes both the light and heavy subunits of acyloxyacyl hydrolase. The encoded enzyme catalyzes the hydrolysis of acyloxylacyl-linked fatty acyl chains from bacterial lipopolysaccharides, effectively detoxifying these molecules. The encoded protein may play a role in modulating host inflammatory response to gram-negative bacteria. Alternatively spliced transcript variants have been described.[provided by RefSeq, Apr 2010]

Uniprot Description

AOAH: Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.77; Secreted, signal peptide; Lipase; Secreted

Chromosomal Location of Human Ortholog: 7p14.2

Cellular Component: extracellular region

Molecular Function: lipoprotein lipase activity; acyloxyacyl hydrolase activity; catalytic activity

Biological Process: lipid metabolic process; inflammatory response