Human Acyloxyacyl Hydrolase (AOAH) ELISA Kit | AOAH elisa kit

Human Acyloxyacyl Hydrolase (AOAH) ELISA Kit

Cat. Num. AAA450261

• Reactivity: Human
• Synonyms: Acyloxyacyl Hydrolase (AOAH); Human Acyloxyacyl Hydrolase (AOAH) ELISA Kit; AOAH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of AOAH. No significant cross-reactivity or interference between AOAH and analogues was observed.
• Sequence Length: 688

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AOAH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AOAH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AOAH elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AOAH in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AOAH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AOAH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AOAH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AOAH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 294610665
• NCBI GeneID: 313
• NCBI Accession #: NP_001170977.1
• NCBI GenBank Nucleotide #: NM_001177506.1
• UniProt Accession #: P28039; Q53F13; A4D1Y5; B7Z490
• Molecular Weight: 61,647 Da
• NCBI Official Full Name: acyloxyacyl hydrolase isoform 2 preproprotein
• NCBI Official Synonym Full Names: acyloxyacyl hydrolase
• NCBI Official Symbol: AOAH
• NCBI Protein Information: acyloxyacyl hydrolase
• UniProt Protein Name: Acyloxyacyl hydrolase
• Protein Family: Acyloxyacyl hydrolase
• UniProt Gene Name: AOAH

NCBI Description

This locus encodes both the light and heavy subunits of acyloxyacyl hydrolase. The encoded enzyme catalyzes the hydrolysis of acyloxylacyl-linked fatty acyl chains from bacterial lipopolysaccharides, effectively detoxifying these molecules. The encoded protein may play a role in modulating host inflammatory response to gram-negative bacteria. Alternatively spliced transcript variants have been described.[provided by RefSeq, Apr 2010]

Uniprot Description

Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides.