Western Blot (WB)
(DENND1B Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DENND1B antibody detected the DENND1B protein (arrow).)
Rabbit DENND1B Polyclonal Antibody | anti-DENND1B antibody
DENND1B Antibody (N-term)
Western Blot (WB)
(DENND1B Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DENND1B antibody detected the DENND1B protein (arrow).)
Western Blot (WB)
(DENND1B Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DENND1B antibody detected the DENND1B protein (arrow).)
Immunohistochemistry (IHC)
(DENND1B antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DENND1B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DENND1B antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DENND1B antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(DENND1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DENND1B Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
mechanism for internalization of proteins and lipids. Members of
the connecdenn family, such as DENND1B, function as guanine
nucleotide exchange factors (GEFs) for the early endosomal small
GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor
protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35
activation with the clathrin machinery (Marat and McPherson, 2010
[PubMed 20154091]).
Sleiman, P.M., et al. N. Engl. J. Med. 362(1):36-44(2010)
Venter, J.C., et al. Science 291(5507):1304-1351(2001)
NCBI and Uniprot Product Information
NCBI Description
Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]
Uniprot Description
DENND1B: a guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Interacts with RAB35. Interacts with clathrin heavy chain/CLTC and with AP2A2. Highly expressed in dendritic and natural killer cells and at lower levels in other myeloid lineage cells and in pituitary. Significantly up-regulated in effector memory T-cells as compared with naive T-cells. Five isoforms of the human protein are produced by alternative splicing. Isoform 5 interacts with AP2B1.
Protein type: GEFs, Rab; GEFs
Chromosomal Location of Human Ortholog: 1q31.3
Cellular Component: clathrin-coated vesicle; cytosol
Molecular Function: Rab guanyl-nucleotide exchange factor activity
Biological Process: protein transport
Research Articles on DENND1B
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Product Notes
The DENND1B dennd1b (Catalog #AAA9211539) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 39-68. The DENND1B Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's DENND1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the DENND1B dennd1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DENND1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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