Rabbit anti-Rat Transforming Growth Factor Beta 1 Induced Transcript 1 (TGFb1I1) Polyclonal Antibody | anti-TGFb1I1 antibody

Polyclonal Antibody to Transforming Growth Factor Beta 1 Induced Transcript 1 (TGFb1I1)

Cat. Num. AAA2015848

• Gene Names: Tgfb1i1; Ara55; Hic-5
• Reactivity: Rat
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Transforming Growth Factor Beta 1 Induced Transcript 1 (TGFb1I1); Polyclonal Antibody; Polyclonal Antibody to Transforming Growth Factor Beta 1 Induced Transcript 1 (TGFb1I1); anti-TGFb1I1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against TGFb1I1. It has been selected for its ability to recognize TGFb1I1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- GCSTT LGGSSFFEKD GAPFCPECYF ERFSPRCGFC NQPIRHKMVT ALGTHWHPEH FCCVSCGEPF GEEGFHEREG RPYCRRDFLQ LFAPRCQGCQ GPILDNYISA LSALWHPDCF VCRECLAPFS GGSFFEHEGR PLCENHFHAQ RGSLCATCGL PVTGRCVSAL GRRFHPDHFT CTFCLRPLTK
• Sequence Length: 444

• Applicable Applications for anti-TGFb1I1 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant TGFb1I1 (Gly256~Gly461) expressed in E.coli.
• Cross Reactivity: Rat
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2079137
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded Bowels tissue))

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Kidney Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 300797521
• NCBI GeneID: 84574
• NCBI Accession #: NP_001178769.1
• NCBI GenBank Nucleotide #: NM_001191840.1
• UniProt Accession #: Q99PD6
• Molecular Weight: 50,123 Da
• NCBI Official Full Name: transforming growth factor beta-1-induced transcript 1 protein
• NCBI Official Synonym Full Names: transforming growth factor beta 1 induced transcript 1
• NCBI Official Symbol: Tgfb1i1
• NCBI Official Synonym Symbols: Ara55; Hic-5
• NCBI Protein Information: transforming growth factor beta-1-induced transcript 1 protein
• UniProt Protein Name: Transforming growth factor beta-1-induced transcript 1 protein
• Protein Family: Transforming growth factor beta-1-induced transcript 1 protein
• UniProt Gene Name: Tgfb1i1
• UniProt Synonym Gene Names: Ara55; Hic-5

NCBI Description

focal adhesion protein/steroid receptor co-activator; binds to hsp27 and inhibits the ability of hsp27 to provide protection against heat shock [RGD, Feb 2006]

Uniprot Description

Hic-5: Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA- binding activity. Homooligomer. Interacts with CRIP2, HSPB1, ILK, LIMS1, LIMS2, NCK2, NUDT16L1, PAK, PPARG, PTPN12, TCF3, TCF7L2 and VCL. Forms a complex with GIT1 and ARHGEF7. Interacts with AR/androgen receptor in a ligand-dependent manner. Interacts with CSK, LYN, MAPK15, NR3C1, PPARG, PTK2/FAK1, PTK2B/PYK2, SLC6A3, SLC6A4, SMAD3, SRC and talin. Up-regulated by TNF and hydrogen peroxide. Expressed in platelets, smooth muscle and prostate stromal cells. Belongs to the paxillin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q37

Cellular Component: cytoplasm; cytoskeleton; extracellular matrix; focal adhesion; intracellular; nuclear matrix

Molecular Function: androgen receptor binding; metal ion binding; protein binding; Roundabout binding; transcription coactivator activity

Biological Process: cell fate commitment; epithelial cell differentiation; morphogenesis of embryonic epithelium; negative regulation of fat cell differentiation; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of transcription, DNA-templated; positive regulation of transforming growth factor beta receptor signaling pathway; response to heat; ubiquitin-dependent SMAD protein catabolic process; Wnt signaling pathway