Western Blot (WB)
(YTHD2 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the YTHD2 antibody detected the YTHD2 protein (arrow).)
Rabbit YTHD2 Polyclonal Antibody | anti-YTHDF2 antibody
YTHD2 Antibody (C-term)
Gene Names
YTHDF2; CAHL; HGRG8; NY-REN-2
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
YTHD2; Polyclonal Antibody; YTHD2 Antibody (C-term); YTH domain-containing family protein 2; CLL-associated antigen KW-14; High-glucose-regulated protein 8; Renal carcinoma antigen NY-REN-2; YTHDF2; HGRG8; anti-YTHDF2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This YTHD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-579 amino acids from the C-terminal region of human YTHD2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
551-579
Sequence Length
529
Applicable Applications for anti-YTHDF2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Other Accession
Q91YT7, Q4R5D9, Q0VCZ3, NP_001166299.1, NP_001166599.1
Function
Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization of to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation.
Cellular Location
Cytoplasm, P-body
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(YTHD2 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the YTHD2 antibody detected the YTHD2 protein (arrow).)
Western Blot (WB)
(YTHD2 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the YTHD2 antibody detected the YTHD2 protein (arrow).)
Related Product Information for anti-YTHDF2 antibody
YTHDF2 is a member of the YTH (YT521-B homology)
superfamily containing YTH domain. The YTH domain is typical for
the eukaryotes and is particularly abundant in plants. The YTH
domain is usually located in the middle of the protein sequence and
may function in binding to RNA. In addition to a YTH domain, this
protein has a proline rich region which may be involved in signal
transduction. An Alu-rich domain has been identified in one of the
introns of this gene, which is thought to be associated with human
longevity. In addition, reciprocal translocations between this gene
and the Runx1 (AML1) gene on chromosme 21 has been observed in
patients with acute myeloid leukemia. This gene was initially
mapped to chromosome 14, which was later turned out to be a
pseudogene.
superfamily containing YTH domain. The YTH domain is typical for
the eukaryotes and is particularly abundant in plants. The YTH
domain is usually located in the middle of the protein sequence and
may function in binding to RNA. In addition to a YTH domain, this
protein has a proline rich region which may be involved in signal
transduction. An Alu-rich domain has been identified in one of the
introns of this gene, which is thought to be associated with human
longevity. In addition, reciprocal translocations between this gene
and the Runx1 (AML1) gene on chromosme 21 has been observed in
patients with acute myeloid leukemia. This gene was initially
mapped to chromosome 14, which was later turned out to be a
pseudogene.
Product Categories/Family for anti-YTHDF2 antibody
References
Nguyen, T.T., et al. Genes Chromosomes Cancer 45(10):918-932(2006)
Cardelli, M., et al. J. Gerontol. A Biol. Sci. Med. Sci. 61(6):547-556(2006)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Stoilov, P., et al. Trends Biochem. Sci. 27(10):495-497(2002)
Scanlan, M.J., et al. Int. J. Cancer 83(4):456-464(1999)
Cardelli, M., et al. J. Gerontol. A Biol. Sci. Med. Sci. 61(6):547-556(2006)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Stoilov, P., et al. Trends Biochem. Sci. 27(10):495-497(2002)
Scanlan, M.J., et al. Int. J. Cancer 83(4):456-464(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62334
NCBI Official Full Name
YTH domain-containing family protein 2 isoform 2
NCBI Official Synonym Full Names
YTH N(6)-methyladenosine RNA binding protein 2
NCBI Official Symbol
YTHDF2
NCBI Official Synonym Symbols
CAHL; HGRG8; NY-REN-2
NCBI Protein Information
YTH domain-containing family protein 2
UniProt Protein Name
YTH domain-containing family protein 2
Protein Family
UniProt Gene Name
YTHDF2
UniProt Synonym Gene Names
HGRG8
UniProt Entry Name
YTHD2_HUMAN
NCBI Description
This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Oct 2012]
Uniprot Description
YTHDF2: high glucose-regulated protein 8. Two alternatively spliced isoforms have been described.
Protein type: RNA processing; RNA-binding
Chromosomal Location of Human Ortholog: 1p35
Biological Process: regulation of mRNA stability; humoral immune response
Disease: Longevity 1
Research Articles on YTHDF2
Similar Products
Product Notes
The YTHDF2 ythdf2 (Catalog #AAA9209250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 551-579. The YTHD2 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Monkey, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's YTHD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the YTHDF2 ythdf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "YTHD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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