Western Blot (WB)
(RNF19B Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RNF19B antibody detected the RNF19B protein (arrow).)
Rabbit RNF19B Polyclonal Antibody | anti-RNF19B antibody
RNF19B Antibody (Center)
Western Blot (WB)
(RNF19B Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RNF19B antibody detected the RNF19B protein (arrow).)
Western Blot (WB)
(RNF19B Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RNF19B antibody detected the RNF19B protein (arrow).)
Flow Cytometry (FC/FACS)
(RNF19B Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RNF19B Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
expressed in natural killer (NK) cells and T lymphocytes following
cytokine stimulation (Kozlowski et al., 1999 [PubMed
10438909]).
Kozlowski, M., et al. J. Immunol. 163(4):1775-1785(1999)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a multi-pass membrane protein containing two RING-type and one IBR-type zinc finger motifs. The encoded protin is an E3 ubiquitin-protein ligase that plays a role in the cytotoxic effects of natural killer (NK) cells. Alternative splicing results in multiple transcript variants. There are pseudogenes for this gene on chromosomes X and Y in a possible pseudoautosomal region. [provided by RefSeq, Jul 2014]
Uniprot Description
RNF19B: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as UCKL1. Involved in the cytolytic activity of natural killer cells and cytotoxic T-cells. Belongs to the RBR family. RNF19 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin ligase; Ligase; EC 6.3.2.-; Membrane protein, integral; Ubiquitin conjugating system; Membrane protein, multi-pass; EC 6.3.2.19
Chromosomal Location of Human Ortholog: 1p35.1
Cellular Component: cytoplasm; integral to membrane
Molecular Function: protein binding; zinc ion binding; ligase activity
Biological Process: natural killer cell mediated cytotoxicity; protein ubiquitination
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Product Notes
The RNF19B rnf19b (Catalog #AAA9207980) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 369-398. The RNF19B Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's RNF19B can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the RNF19B rnf19b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF19B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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