Rabbit anti-Human RNF19B Polyclonal Antibody | anti-RNF19B antibody

RNF19B, ID (RNF19B, IBRDC3, NKLAM, E3 ubiquitin-protein ligase RNF19B, IBR domain-containing protein 3, Natural killer lytic-associated molecule, RING finger protein 19B) (Biotin)

Cat. Num. AAA6342777

• Gene Names: RNF19B; NKLAM; IBRDC3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNF19B; Polyclonal Antibody; ID (RNF19B; IBRDC3; NKLAM; E3 ubiquitin-protein ligase RNF19B; IBR domain-containing protein 3; Natural killer lytic-associated molecule; RING finger protein 19B) (Biotin); RING finger protein 19B; anti-RNF19B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RNF19B antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RNF19B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-405 amino acids from the Central region of human RNF19B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RNF19B Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RNF19B antibody detected the RNF19B protein (arrow).)

Flow Cytometry (FC/FACS)

(RNF19B Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RNF19B antibody

IBRDC3 is a cytolysis-associated transmembrane protein expressed in natural killer (NK) cells and T lymphocytes following cytokine stimulation (Kozlowski et al., 1999 [PubMed 10438909]).

Product Categories/Family for anti-RNF19B antibody

Antibodies; Ubiquitin Ligases

References

Fortier, J.M., et al. J. Immunol. 176(11):6454-6463(2006). Kozlowski, M., et al. J. Immunol. 163(4):1775-1785(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 188219579
• NCBI GeneID: 127544
• NCBI Accession #: NP_001120833.1; NP_699172.2
• NCBI GenBank Nucleotide #: NM_001127361.2
• UniProt Accession #: Q6ZMZ0; Q0VG77; Q5TH44; Q5TH45; Q6P6A4; Q8N2S8; Q8WUF3; B7ZLB2; E9PAW6; G3XA82
• Molecular Weight: 77,925 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF19B isoform b
• NCBI Official Synonym Full Names: ring finger protein 19B
• NCBI Official Symbol: RNF19B
• NCBI Official Synonym Symbols: NKLAM; IBRDC3
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF19B; IBR domain-containing protein 3; natural killer lytic-associated molecule
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF19B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF19B
• UniProt Synonym Gene Names: IBRDC3; NKLAM
• UniProt Entry Name: RN19B_HUMAN