Rabbit anti-Human PLB-T17 Polyclonal Antibody | anti-PLN antibody

PLB-T17 Antibody

Cat. Num. AAA9205753

• Gene Names: PLN; PLB; CMD1P; CMH18
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: PLB-T17; Polyclonal Antibody; PLB-T17 Antibody; Cardiac phospholamban; PLB; PLN; anti-PLN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PLB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human PLB.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.34 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 52

• Applicable Applications for anti-PLN antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of PLB-T17 in Jurkat cell line lysates (35ug/lane). PLB (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PLB-T17 Antibody IHC analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLB-T17 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PLB-T17 Antibody flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PLN antibody

PLB is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.

Product Categories/Family for anti-PLN antibody

Cardiovascular; Metabolism; Signal transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4505887
• NCBI GeneID: 5350
• NCBI Accession #: NP_002658.1
• NCBI GenBank Nucleotide #: NM_002667.3
• UniProt Accession #: P26678
• Molecular Weight: 6109
• NCBI Official Full Name: cardiac phospholamban
• NCBI Official Synonym Full Names: phospholamban
• NCBI Official Symbol: PLN
• NCBI Official Synonym Symbols: PLB; CMD1P; CMH18
• NCBI Protein Information: cardiac phospholamban
• UniProt Protein Name: Cardiac phospholamban
• Protein Family: Cardiac phospholamban
• UniProt Gene Name: PLN
• UniProt Synonym Gene Names: PLB; PLB
• UniProt Entry Name: PPLA_HUMAN

NCBI Description

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008]

Uniprot Description

PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

Protein type: Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: sarcoplasmic reticulum membrane; mitochondrion; membrane; endoplasmic reticulum; perinuclear region of cytoplasm; mitochondrial membrane; vesicle

Molecular Function: enzyme inhibitor activity; identical protein binding; protein binding; ATPase inhibitor activity; calcium channel regulator activity; ATPase binding

Biological Process: cardiac muscle development; negative regulation of calcium ion transport; negative regulation of heart rate; cytosolic calcium ion homeostasis; blood circulation; regulation of calcium ion transport; response to testosterone stimulus; regulation of heart contraction; response to insulin stimulus; response to zinc ion; calcium ion transport; negative regulation of catalytic activity; negative regulation of ATPase activity; protein homooligomerization; regulation of the force of heart contraction

Disease: Cardiomyopathy, Dilated, 1p; Cardiomyopathy, Familial Hypertrophic, 18