Western Blot (WB)
(Western blot analysis of XDH Antibody (N-term) in mouse lung tissue lysates (35ug/lane). XDH (arrow) was detected using the purified Pab.)
Rabbit anti-Human, mouse XDH Polyclonal Antibody | anti-XDH antibody
XDH Antibody (N-term)
Western Blot (WB)
(Western blot analysis of XDH Antibody (N-term) in mouse lung tissue lysates (35ug/lane). XDH (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of XDH Antibody (N-term) in mouse lung tissue lysates (35ug/lane). XDH (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with XDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with XDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(XDH Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(XDH Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Taibi,G., et.al., J. Cell. Biochem. 108 (3), 688-692 (2009)
Spiekermann,S., et.al., Eur. Respir. J. 34 (1), 276 (2009)
NCBI and Uniprot Product Information
NCBI Description
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]
Uniprot Description
XDH: Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). Defects in XDH are the cause of xanthinuria type 1 (XU1). Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. Belongs to the xanthine dehydrogenase family.
Protein type: Oxidoreductase; EC 1.17.3.2; Secondary Metabolites Metabolism - caffeine; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 1.17.1.4; Nucleotide Metabolism - purine
Chromosomal Location of Human Ortholog: 2p23.1
Cellular Component: extracellular space; peroxisome; cytosol
Molecular Function: molybdopterin cofactor binding; 2 iron, 2 sulfur cluster binding; UDP-N-acetylmuramate dehydrogenase activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors; protein homodimerization activity; electron carrier activity; FAD binding; iron ion binding; xanthine dehydrogenase activity; xanthine oxidase activity
Biological Process: lactation; caspase activation; xanthine catabolic process; negative regulation of protein kinase B signaling cascade; negative regulation of endothelial cell differentiation; negative regulation of protein amino acid phosphorylation; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of endothelial cell proliferation; purine nucleotide catabolic process; purine base metabolic process
Disease: Xanthinuria, Type I
Research Articles on XDH
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Product Notes
The XDH xdh (Catalog #AAA9204781) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 206-234. The XDH Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's XDH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the XDH xdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XDH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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