Immunofluorescence (IF)
(Fluorescent image of SH-SY5Y cells stained with SUMO2/3 Antibody (C-term). MBS9202693 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Rabbit SUMO2/3 Polyclonal Antibody | anti-SUMO3 antibody
SUMO2/3 Antibody (C-term)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of SH-SY5Y cells stained with SUMO2/3 Antibody (C-term). MBS9202693 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of SH-SY5Y cells stained with SUMO2/3 Antibody (C-term). MBS9202693 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with SUMO2/3 Antibody (C-term). MBS9202693 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of Hela cells stained with SUMO2/3 Antibody (C-term). MBS9202693 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with SUMO2/3 Antibody(C-term). MBS9202693 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with SUMO2/3 Antibody(C-term). MBS9202693 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Western Blot (WB)
(Western blot analysis of lysates from 293T, Hela, HL-60, Jurkat cell lines and rat liver tissue lysate (from left to right), using SUMO2/3 Antibody (Q65). MBS9202693 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysates from 293T, Hela, HL-60, Jurkat cell lines and rat liver tissue lysate (from left to right), using SUMO2/3 Antibody (Q65). MBS9202693 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Western Blot (WB)
(SUMO2/3 Antibody (C-term) western blot analysis in U251 cell lysate,mouse liver and rat liver tissue lysates (35ug/lane). This demonstrates that the SUMO2/3 antibody detected SUMO2/3 protein (arrow).)
Western Blot (WB)
(SUMO2/3 Antibody (C-term) western blot analysis in U251 cell lysate,mouse liver and rat liver tissue lysates (35ug/lane). This demonstrates that the SUMO2/3 antibody detected SUMO2/3 protein (arrow).)
Western Blot (WB)
(SUMO2/3 Antibody (C-term) western blot analysis in Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).)
Western Blot (WB)
(SUMO2/3 Antibody (C-term) western blot analysis in Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).)
Western Blot (WB)
(The anti-SUMO2/3 C-term Pab is used in Western blot to detect SUMO2/3 in HeLa cell lysate.)
Western Blot (WB)
(The anti-SUMO2/3 C-term Pab is used in Western blot to detect SUMO2/3 in HeLa cell lysate.)
Lapenta, V., et al., Genomics 40(2):362-366 (1997).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Feb 2014]
Uniprot Description
SUMO3: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Covalently attached to a number of proteins. Interacts with USP25 (via ts SIM domain); the interaction sumoylates USP25 and inhibits its ubiquitin hydrolyzing activity. Interacts with SAE2 and UBE2I. Expressed predominantly in liver. Belongs to the ubiquitin family. SUMO subfamily.
Protein type: Ubiquitin-like modifier
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: nucleoplasm; kinetochore; PML body; cytoplasm; nucleus
Molecular Function: protein binding; SUMO ligase activity
Biological Process: cellular protein metabolic process; protein sumoylation; post-translational protein modification
Research Articles on SUMO3
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Product Notes
The SUMO3 sumo3 (Catalog #AAA9202693) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 49-81. The SUMO2/3 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Xenopus, Zebrafish, Bovine, Chicken, Hamster, Monkey, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's SUMO2/3 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). IF~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the SUMO3 sumo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SUMO2/3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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