Rabbit anti-Human, Mouse SUMO2/3 Polyclonal Antibody | anti-SUMO2/3 antibody

SUMO2/3, CT (SUMO3, SMT3B, SMT3H1, Small ubiquitin-related modifier 3, SMT3 homolog 1, SUMO-2, Ubiquitin-like protein SMT3B) (Azide free) (HRP)

Cat. Num. AAA6352614

• Gene Names: SUMO3; SMT3A; Smt3B; SMT3H1; SUMO-3
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SUMO2/3; Polyclonal Antibody; CT (SUMO3; SMT3B; SMT3H1; Small ubiquitin-related modifier 3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B) (Azide free) (HRP); SUMO3; Ubiquitin-like protein SMT3B; anti-SUMO2/3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-SUMO2/3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids within C-terminal region of human SUMO2/3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-SUMO2/3 C-term Pab is used in Western blot to detect SUMO2/3 in HeLa cell lysate.)

Western Blot (WB)

(Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (Q65). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-SUMO2/3 antibody

SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.

Product Categories/Family for anti-SUMO2/3 antibody

Antibodies; Ubiquitin

References

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Lapenta, V., et al., Genomics 40(2):362-366 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 23503102
• NCBI GeneID: 6612
• UniProt Accession #: P55854; Q53HI9; Q6FGD4; Q9BWR4; B2R5X4; B4DUW4
• Molecular Weight: 103
• NCBI Official Full Name: Small ubiquitin-related modifier 3
• NCBI Official Synonym Full Names: small ubiquitin-like modifier 3
• NCBI Official Symbol: SUMO3
• NCBI Official Synonym Symbols: SMT3A; Smt3B; SMT3H1; SUMO-3
• NCBI Protein Information: small ubiquitin-related modifier 3; ubiquitin-like protein SMT3B; SMT3 suppressor of mif two 3 homolog 1; SMT3 suppressor of mif two 3 homolog 3
• UniProt Protein Name: Small ubiquitin-related modifier 3
• UniProt Gene Name: SUMO3
• UniProt Synonym Gene Names: SUMO-3Curated
• UniProt Entry Name: SUMO3_HUMAN

NCBI Description

This gene encodes a member of the small ubiquitin-related modifier (SUMO) family of eukaryotic proteins. The encoded protein is covalently conjugated to other proteins via a post-translation modification known as sumoylation. Sumoylation may play a role in a wide variety of cellular processes, including nuclear transport, DNA replication and repair, mitosis, transcriptional regulation, and signal transduction. Alternatively spliced transcript variants encoding distinct proteins have been described. [provided by RefSeq, Feb 2014]

Uniprot Description

SUMO3: Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Covalently attached to a number of proteins. Interacts with USP25 (via ts SIM domain); the interaction sumoylates USP25 and inhibits its ubiquitin hydrolyzing activity. Interacts with SAE2 and UBE2I. Expressed predominantly in liver. Belongs to the ubiquitin family. SUMO subfamily.

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: kinetochore; nucleoplasm; PML body; cytoplasm; nucleus

Molecular Function: protein binding; SUMO ligase activity

Biological Process: cellular protein metabolic process; protein sumoylation; post-translational protein modification