Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ADIPOR2 Antibody (Center) (MBS9201931) with Hela cell followed by Alexa Fluor489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human ADIPOR2 Polyclonal Antibody | anti-ADIPOR2 antibody
ADIPOR2 Antibody (Center)
WB: 1:1000
FC: 1:25
Note=Localized to the cell membrane and intracellular organelles
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ADIPOR2 Antibody (Center) (MBS9201931) with Hela cell followed by Alexa Fluor489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of ADIPOR2 Antibody (Center) (MBS9201931) with Hela cell followed by Alexa Fluor489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB)
(Western blot analysis of ADIPOR2 Antibody (Center) (MBS9201931) in Hela cell line lysates (35ug/lane). ADIPOR2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ADIPOR2 Antibody (Center) (MBS9201931) in Hela cell line lysates (35ug/lane). ADIPOR2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(All lanes : Anti-ADIPOR2 Antibody (Center) at 1:2000 dilutionLane 1: U-251 MG whole cell lysateLane 2: U-2OS whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-ADIPOR2 Antibody (Center) at 1:2000 dilutionLane 1: U-251 MG whole cell lysateLane 2: U-2OS whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human liver tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human liver tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human skeletal muscle tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-ADIPOR2 Antibody (Center) at 1:1000 dilution + Human skeletal muscle tissue lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Flow Cytometry (FC)
(Overlay histogram showing U-2 OS cells stained with MBS9201931 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9201931, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry (FC)
(Overlay histogram showing U-2 OS cells stained with MBS9201931 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9201931, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin.
Function:
Receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism (PubMed: 12802337, PubMed: 25855295). Required for normal body fat and glucose homeostasis. ADIPOQ-binding activates a signaling cascade that leads to increased PPARA activity, and ultimately to increased fatty acid oxidation and glucose uptake. Has intermediate affinity for globular and full-length adiponectin. Required for normal revascularization after chronic ischemia caused by severing of blood vessels (By similarity).
NCBI and Uniprot Product Information
NCBI Description
The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM, Mar 2008]
Uniprot Description
ADIPOR2: Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin. Belongs to the ADIPOR family.
Protein type: Receptor, misc.; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: integral to membrane; plasma membrane
Molecular Function: adiponectin binding; identical protein binding; hormone binding; protein heterodimerization activity; receptor activity
Biological Process: positive regulation of glucose import; hormone-mediated signaling; heart development; adiponectin-mediated signaling pathway; fatty acid oxidation; female pregnancy; negative regulation of cell growth; response to nutrient
Research Articles on ADIPOR2
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Product Notes
The ADIPOR2 adipor2 (Catalog #AAA9201931) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 45-72. The ADIPOR2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADIPOR2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF), Flow Cytometry (FC/FACS). IF: 1:10~50 WB: 1:1000 FC: 1:25. Researchers should empirically determine the suitability of the ADIPOR2 adipor2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADIPOR2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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