Western Blot (WB)
(The anti-PFKFB4 Pab is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.)
Rabbit PFKFB4 Polyclonal Antibody | anti-PFKFB4 antibody
PFKFB4 Antibody (Center)
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PFKFB4; Polyclonal Antibody; PFKFB4 Antibody (Center); 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 4; 6PF-2-K/Fru-2; 6-P2ase 4; PFK/FBPase 4; 6-P2ase testis-type isozyme; 6-phosphofructo-2-kinase; Fructose-2; 6-bisphosphatase; anti-PFKFB4 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PFKFB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-296 amino acids from the Central region of human PFKFB4.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
266-296
Sequence Length
435
Applicable Applications for anti-PFKFB4 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(The anti-PFKFB4 Pab is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.)
Western Blot (WB)
(The anti-PFKFB4 Pab is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB4 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB4 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Related Product Information for anti-PFKFB4 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
Product Categories/Family for anti-PFKFB4 antibody
References
Sakai, A., et al., J. Biochem. 119(3):506-511 (1996). Manzano, A., et al., Gene 229 (1-2), 83-89 (1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54040
NCBI Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
NCBI Official Synonym Full Names
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
NCBI Official Symbol
PFKFB4
NCBI Protein Information
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
UniProt Protein Name
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
Protein Family
UniProt Gene Name
PFKFB4
UniProt Synonym Gene Names
6PF-2-K/Fru-2,6-P2ase 4; PFK/FBPase 4
UniProt Entry Name
F264_HUMAN
Uniprot Description
PFKFB4: Synthesis and degradation of fructose 2,6-bisphosphate.
Protein type: Motility/polarity/chemotaxis; EC 2.7.1.105; Kinase, other; Phosphatase (non-protein); EC 3.1.3.46; Carbohydrate Metabolism - fructose and mannose
Chromosomal Location of Human Ortholog: 3p22-p21
Cellular Component: cytosol
Molecular Function: fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; ATP binding
Biological Process: glycolysis; dephosphorylation; carbohydrate metabolic process; carbohydrate phosphorylation; glucose metabolic process; pathogenesis; fructose 2,6-bisphosphate metabolic process; fructose metabolic process
Research Articles on PFKFB4
Similar Products
Product Notes
The PFKFB4 pfkfb4 (Catalog #AAA9201289) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 266-296. The PFKFB4 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's PFKFB4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the PFKFB4 pfkfb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PFKFB4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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