Rabbit anti-Human, Mouse PFKFB4 Polyclonal Antibody | anti-PFKFB4 antibody

PFKFB4, ID (PFKFB4, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4, 6PF-2-K/Fru-2,6-P2ase testis-type isozyme, 6-phosphofructo-2-kinase, Fructose-2,6-bisphosphatase) (PE)

Cat. Num. AAA6332891

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PFKFB4; Polyclonal Antibody; ID (PFKFB4; 6-phosphofructo-2-kinase/fructose-2; 6-bisphosphatase 4; 6PF-2-K/Fru-2; 6-P2ase testis-type isozyme; 6-phosphofructo-2-kinase; Fructose-2; 6-bisphosphatase) (PE); 6-bisphosphatase; anti-PFKFB4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PFKFB4 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PFKFB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274~303 amino acids from the center region of human PFKFB4.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(The anti-PFKFB4 Pab is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB4 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-PFKFB4 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Product Categories/Family for anti-PFKFB4 antibody

Antibodies; Protein Kinases

References

Sakai, A., et al., J. Biochem. 119(3):506-511 (1996). Manzano, A., et al., Gene 229 (1-2), 83-89 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 19923257
• NCBI GeneID: 5210
• NCBI Accession #: NP_004558.1
• NCBI GenBank Nucleotide #: NM_004567
• UniProt Accession #: Q16877; Q5S3G5
• Molecular Weight: ~82 kDa
• NCBI Official Full Name: Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 (PFKFB4), mRNA
• NCBI Official Synonym Full Names: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
• NCBI Official Symbol: PFKFB4
• NCBI Protein Information: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4; PFK/FBPase 4; 6PF-2-K/Fru-2,6-P2ase 4; 6PF-2-K/Fru-2,6-P2ase testis-type isozyme; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-4; bifunctional enzyme with kinase and biphosphatase activities
• UniProt Protein Name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4
• Protein Family: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
• UniProt Gene Name: PFKFB4
• UniProt Synonym Gene Names: 6PF-2-K/Fru-2,6-P2ase 4
• UniProt Entry Name: F264_HUMAN

Uniprot Description

Function: Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activity: Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Enzyme regulation: The most important regulatory mechanism of these opposing activities is by phosphorylation and dephosphorylation of the enzyme

By similarity.

Subunit structure: Homodimer

By similarity.

Sequence similarities: In the C-terminal section; belongs to the phosphoglycerate mutase family.