Western Blot (WB)
(Western blot analysis of RAT SERPH Antibody (Center) in rat heart tissue lysates (35ug/lane).SERPH (arrow) was detected using the purified Pab.(1:100))
Mouse Serpinh1 Monoclonal Antibody | anti-Serpinh1 antibody
Rat Serpinh1 Antibody (Center)
Gene Names
Serpinh1; Cbp2; Serpinh2
Reactivity
Human, rat (Predicted Reactivity: Bovine, Mouse)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
Serpinh1; Monoclonal Antibody; Rat Serpinh1 Antibody (Center); Serpin H1; 47 kDa heat shock protein; Collagen-binding protein; Colligin; GP46; Cbp1; Hsp47; anti-Serpinh1 antibody
Host
Mouse
Reactivity
Human, rat (Predicted Reactivity: Bovine, Mouse)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
134CT3.1.1
Specificity
This Rat Serpinh1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 222-251 amino acids from the Central region of rat Serpinh1.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.35 (varies by lot)
Sequence Positions
222-251
Sequence Length
417
Applicable Applications for anti-Serpinh1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:100~200
IHC~~1:50~100
IHC~~1:50~100
Antigen Type
Synthetic Peptide
Antigen Source
RAT
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of RAT SERPH Antibody (Center) in rat heart tissue lysates (35ug/lane).SERPH (arrow) was detected using the purified Pab.(1:100))
Western Blot (WB)
(Western blot analysis of RAT SERPH Antibody (Center) in rat heart tissue lysates (35ug/lane).SERPH (arrow) was detected using the purified Pab.(1:100))
Immunohistochemistry (IHC)
(RAT SERPH Monoclonal Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RAT SERPH Monoclonal Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RAT SERPH Monoclonal Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RAT SERPH Monoclonal Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
References
Localization of HSP47 mRNA in murine bleomycin-induced pulmonary fibrosis. Kakugawa T, et al. Virchows Arch, 2010 Mar. PMID 20094730. The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis. Sansom SN, et al. PLoS Genet, 2009 Jun. PMID 19521500. Fog2 is critical for cardiac function and maintenance of coronary vasculature in the adult mouse heart. Zhou B, et al. J Clin Invest, 2009 Jun. PMID 19411759. Adenovirus-mediated RNA interference against collagen-specific molecular chaperone 47-KDa heat shock protein suppresses scar formation on mouse wounds. Wang Z, et al. Cell Biol Int, 2008 May. PMID 18093850. Type I collagen in Hsp47-null cells is aggregated in endoplasmic reticulum and deficient in N-propeptide processing and fibrillogenesis. Ishida Y, et al. Mol Biol Cell, 2006 May. PMID 16525016.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46518
NCBI Official Full Name
serpin H1
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
NCBI Official Symbol
Serpinh1
NCBI Official Synonym Symbols
Cbp2; Serpinh2
NCBI Protein Information
serpin H1
UniProt Protein Name
Serpin H1
Protein Family
UniProt Gene Name
Serpinh1
UniProt Synonym Gene Names
Cbp1; Hsp47; Colligin
UniProt Entry Name
SERPH_RAT
NCBI Description
binds collagen; may act as a molecular chaperone [RGD, Feb 2006]
Uniprot Description
SERPINH1: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10). A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae. Belongs to the serpin family.
Cellular Component: extracellular space; endoplasmic reticulum; endoplasmic reticulum lumen; cytoplasm; ER-Golgi intermediate compartment
Molecular Function: collagen binding; serine-type endopeptidase inhibitor activity; unfolded protein binding
Biological Process: collagen fibril organization; protein maturation; collagen biosynthetic process
Research Articles on Serpinh1
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Product Notes
The Serpinh1 serpinh1 (Catalog #AAA9200175) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 222-251. The Rat Serpinh1 Antibody (Center) reacts with Human, rat (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Serpinh1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:100~200 IHC~~1:50~100. Researchers should empirically determine the suitability of the Serpinh1 serpinh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serpinh1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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